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Assay Detail

CHEMBL3364610

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-tagged PLK4 (1 to 391 residues) expressed in Escherichia coli incubated for 30 mins by ELISA method
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PLK4 (CHEMBL3788)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of Polo-like kinase 4 inhibitors: design and optimization of spiro[cyclopropane-1,3'[3H]indol]-2'(1'H).ones as orally bioavailable antitumor agents.
Sampson PB, Liu Y, Patel NK, Feher M, Forrest B, Li SW, Edwards L, Laufer R, Lang Y, Ban F, Awrey DE, Mao G, Plotnikova O, Leung G, Hodgson R, Mason JM, Wei X, Kiarash R, Green E, Qiu W, Chirgadze NY, Mak TW, Pan G, Pauls HW.
J Med Chem (2015) 58 : 130 -146
PubMed 24867403 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 8.42 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353351 1 9.15
CHEMBL3353352 1 9.10
CHEMBL3353348 1 8.96
CHEMBL3353350 1 8.92
CHEMBL3353361 1 8.89
CHEMBL3353346 1 8.85
CHEMBL3353347 1 8.85
CHEMBL3353342 1 8.80
CHEMBL3353360 1 8.74
CHEMBL3353345 1 8.70
CHEMBL3353353 1 8.70
CHEMBL3353341 1 8.66
CHEMBL3353355 1 8.66
CHEMBL3353354 1 8.64
CHEMBL3353359 1 8.62
CHEMBL2407749 1 8.62
CHEMBL3353349 1 8.59
CHEMBL3353358 1 8.47
CHEMBL3353340 1 8.44
CHEMBL2407897 1 8.40
CHEMBL3353343 1 8.34
CHEMBL3353356 1 8.31
CHEMBL3353344 1 8.20
CHEMBL3353362 1 8.17
CHEMBL3353357 1 8.14
CHEMBL2407900 1 8.14
CHEMBL1289926 AXITINIB 4.0 1 7.37
CHEMBL3353337 1 6.64
CHEMBL3353338 1 6.00
CHEMBL3353339 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353351 IC50 = 0.71 nM 9.15
CHEMBL3353352 IC50 = 0.79 nM 9.10
CHEMBL3353348 IC50 = 1.1 nM 8.96
CHEMBL3353350 IC50 = 1.2 nM 8.92
CHEMBL3353361 IC50 = 1.3 nM 8.89
CHEMBL3353346 IC50 = 1.4 nM 8.85
CHEMBL3353347 IC50 = 1.4 nM 8.85
CHEMBL3353342 IC50 = 1.6 nM 8.80
CHEMBL3353360 IC50 = 1.8 nM 8.74
CHEMBL3353345 IC50 = 2.0 nM 8.70
CHEMBL3353353 IC50 = 2.0 nM 8.70
CHEMBL3353341 IC50 = 2.2 nM 8.66
CHEMBL3353355 IC50 = 2.2 nM 8.66
CHEMBL3353354 IC50 = 2.3 nM 8.64
CHEMBL3353359 IC50 = 2.4 nM 8.62
CHEMBL2407749 IC50 = 2.4 nM 8.62
CHEMBL3353349 IC50 = 2.6 nM 8.59
CHEMBL3353358 IC50 = 3.4 nM 8.47
CHEMBL3353340 IC50 = 3.6 nM 8.44
CHEMBL2407897 IC50 = 4.0 nM 8.40
CHEMBL3353343 IC50 = 4.6 nM 8.34
CHEMBL3353356 IC50 = 4.9 nM 8.31
CHEMBL3353344 IC50 = 6.3 nM 8.20
CHEMBL3353362 IC50 = 6.7 nM 8.17
CHEMBL3353357 IC50 = 7.2 nM 8.14
CHEMBL2407900 IC50 = 7.3 nM 8.14
CHEMBL1289926 AXITINIB IC50 = 43.0 nM 7.37
CHEMBL3353337 IC50 = 230.0 nM 6.64
CHEMBL3353338 IC50 = 1000.0 nM 6.00
CHEMBL3353339 IC50 > 50000.0 nM -