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Assay Detail

CHEMBL3366862

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kbeta in human MAD-MB-468 cells assessed as inhibition of Ser473 Akt phosphorylation by cellular potency assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of 9-(1-anilinoethyl)-2-morpholino-4-oxo-pyrido[1,2-a]pyrimidine-7-carboxamides as PI3Kβ/δ inhibitors for the treatment of PTEN-deficient tumours.
Barlaam B, Cosulich S, Degorce S, Fitzek M, Giordanetto F, Green S, Inghardt T, Hennequin L, Hancox U, Lambert-van der Brempt C, Morgentin R, Pass S, Plé P, Saleh T, Ward L.
Bioorg Med Chem Lett (2014) 24 : 3928 -3935
PubMed 24992874 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.62 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3319646 1 8.70
CHEMBL3319490 1 8.52
CHEMBL3319485 1 8.40
CHEMBL3319644 1 8.15
CHEMBL3319494 1 8.10
CHEMBL3318715 1 8.10
CHEMBL3319642 1 8.05
CHEMBL3319643 1 8.00
CHEMBL3319492 1 7.96
CHEMBL3319491 1 7.96
CHEMBL3319645 1 7.92
CHEMBL3319641 1 7.77
CHEMBL1972466 1 7.66
CHEMBL3319647 1 7.64
CHEMBL3319493 1 7.60
CHEMBL3319495 1 7.60
CHEMBL3319496 1 7.54
CHEMBL3319489 1 7.47
CHEMBL3319487 1 7.41
CHEMBL2165191 AZD-6482 2.0 1 7.40
CHEMBL3319486 1 7.21
CHEMBL3319488 1 7.14
CHEMBL3319648 1 7.13
CHEMBL3317473 1 7.12
CHEMBL3319639 1 7.09
CHEMBL3318711 1 6.81
CHEMBL3319640 1 6.72
CHEMBL3319649 1 6.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3319646 IC50 = 2.0 nM 8.70
CHEMBL3319490 IC50 = 3.0 nM 8.52
CHEMBL3319485 IC50 = 4.0 nM 8.40
CHEMBL3319644 IC50 = 7.0 nM 8.15
CHEMBL3319494 IC50 = 8.0 nM 8.10
CHEMBL3318715 IC50 = 8.0 nM 8.10
CHEMBL3319642 IC50 = 9.0 nM 8.05
CHEMBL3319643 IC50 = 10.0 nM 8.00
CHEMBL3319492 IC50 = 11.0 nM 7.96
CHEMBL3319491 IC50 = 11.0 nM 7.96
CHEMBL3319645 IC50 = 12.0 nM 7.92
CHEMBL3319641 IC50 = 17.0 nM 7.77
CHEMBL1972466 IC50 = 22.0 nM 7.66
CHEMBL3319647 IC50 = 23.0 nM 7.64
CHEMBL3319493 IC50 = 25.0 nM 7.60
CHEMBL3319495 IC50 = 25.0 nM 7.60
CHEMBL3319496 IC50 = 29.0 nM 7.54
CHEMBL3319489 IC50 = 34.0 nM 7.47
CHEMBL3319487 IC50 = 39.0 nM 7.41
CHEMBL2165191 AZD-6482 IC50 = 40.0 nM 7.40
CHEMBL3319486 IC50 = 62.0 nM 7.21
CHEMBL3319488 IC50 = 73.0 nM 7.14
CHEMBL3319648 IC50 = 74.0 nM 7.13
CHEMBL3317473 IC50 = 75.0 nM 7.12
CHEMBL3319639 IC50 = 81.0 nM 7.09
CHEMBL3318711 IC50 = 156.0 nM 6.81
CHEMBL3319640 IC50 = 190.0 nM 6.72
CHEMBL3319649 IC50 = 530.0 nM 6.28