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Assay Detail

CHEMBL3369493

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-inflammatory activity in LPS-stimulated mouse RAW264.7 cells assessed as suppression of TNFalpha production pre-incubated for 1 hr before LPS stimulation for 24 hrs by ELISA method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Indole-3-ethylsulfamoylphenylacrylamides: potent histone deacetylase inhibitors with anti-inflammatory activity.
Mehndiratta S, Hsieh YL, Liu YM, Wang AW, Lee HY, Liang LY, Kumar S, Teng CM, Yang CR, Liou JP.
Eur J Med Chem (2014) 85 : 468 -479
PubMed 25113875 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.07 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3330002 1 6.96
CHEMBL3329991 1 6.32
CHEMBL3329999 1 6.21
CHEMBL3329992 1 6.14
CHEMBL3329990 1 6.10
CHEMBL3329998 1 5.92
CHEMBL3329993 1 5.82
CHEMBL3329994 1 5.82
CHEMBL408513 BELINOSTAT 4.0 1 5.33
CHEMBL3329995 1 -
CHEMBL3329996 1 -
CHEMBL3329997 1 -
CHEMBL3330000 1 -
CHEMBL3330001 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3330002 IC50 = 110.0 nM 6.96
CHEMBL3329991 IC50 = 480.0 nM 6.32
CHEMBL3329999 IC50 = 620.0 nM 6.21
CHEMBL3329992 IC50 = 730.0 nM 6.14
CHEMBL3329990 IC50 = 800.0 nM 6.10
CHEMBL3329998 IC50 = 1200.0 nM 5.92
CHEMBL3329993 IC50 = 1500.0 nM 5.82
CHEMBL3329994 IC50 = 1500.0 nM 5.82
CHEMBL408513 BELINOSTAT IC50 = 4700.0 nM 5.33
CHEMBL3329995 IC50 > 10000.0 nM -
CHEMBL3329996 IC50 > 10000.0 nM -
CHEMBL3329997 IC50 > 10000.0 nM -
CHEMBL3330000 IC50 > 10000.0 nM -
CHEMBL3330001 IC50 > 10000.0 nM -