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Assay Detail

CHEMBL3369647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of c-Met autophosphorylation in human NCI-H441 cells for 1 hr by ELISA
31
Total Activities
25
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NCI-H441
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hepatocyte growth factor receptor (CHEMBL3717)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (S)-1-(1-(Imidazo[1,2-a]pyridin-6-yl)ethyl)-6-(1-methyl-1H-pyrazol-4-yl)-1H-[1,2,3]triazolo[4,5-b]pyrazine (volitinib) as a highly potent and selective mesenchymal-epithelial transition factor (c-Met) inhibitor in clinical development for treatment of cancer.
Jia H, Dai G, Weng J, Zhang Z, Wang Q, Zhou F, Jiao L, Cui Y, Ren Y, Fan S, Zhou J, Qing W, Gu Y, Wang J, Sai Y, Su W.
J Med Chem (2014) 57 : 7577 -7589
PubMed 25148209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.76 8.52
Inhibition 6 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3334569 1 8.52
CHEMBL3334567 SAVOLITINIB 3.0 1 8.52
CHEMBL2170816 1 8.22
CHEMBL3334557 2 8.22
CHEMBL3334540 1 8.15
CHEMBL3334551 2 8.15
CHEMBL3334552 2 8.10
CHEMBL3334556 2 8.00
CHEMBL3334561 2 8.00
CHEMBL3334542 2 7.96
CHEMBL3334565 1 7.92
CHEMBL3334564 1 7.92
CHEMBL3334541 1 7.72
CHEMBL3334546 1 7.70
CHEMBL3334549 1 7.58
CHEMBL3334563 1 7.58
CHEMBL3334570 1 7.57
CHEMBL3334562 1 7.51
CHEMBL3334568 1 7.50
CHEMBL3334550 1 7.48
CHEMBL3334560 1 7.21
CHEMBL3334545 1 7.20
CHEMBL2431841 1 7.16
CHEMBL3334544 1 7.11
CHEMBL3334553 1 6.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3334567 SAVOLITINIB IC50 = 3.0 nM 8.52
CHEMBL3334569 IC50 = 3.0 nM 8.52
CHEMBL2170816 IC50 = 6.0 nM 8.22
CHEMBL3334557 IC50 = 6.0 nM 8.22
CHEMBL3334540 IC50 = 7.0 nM 8.15
CHEMBL3334551 IC50 = 7.0 nM 8.15
CHEMBL3334552 IC50 = 8.0 nM 8.10
CHEMBL3334561 IC50 = 10.0 nM 8.00
CHEMBL3334556 IC50 = 10.0 nM 8.00
CHEMBL3334542 IC50 = 11.0 nM 7.96
CHEMBL3334565 IC50 = 12.0 nM 7.92
CHEMBL3334564 IC50 = 12.0 nM 7.92
CHEMBL3334541 IC50 = 19.0 nM 7.72
CHEMBL3334546 IC50 = 20.0 nM 7.70
CHEMBL3334563 IC50 = 26.0 nM 7.58
CHEMBL3334549 IC50 = 26.0 nM 7.58
CHEMBL3334570 IC50 = 27.0 nM 7.57
CHEMBL3334562 IC50 = 31.0 nM 7.51
CHEMBL3334568 IC50 = 32.0 nM 7.50
CHEMBL3334550 IC50 = 33.0 nM 7.48
CHEMBL3334560 IC50 = 61.0 nM 7.21
CHEMBL3334545 IC50 = 63.0 nM 7.20
CHEMBL2431841 IC50 = 69.0 nM 7.16
CHEMBL3334544 IC50 = 77.0 nM 7.11
CHEMBL3334553 IC50 = 120.0 nM 6.92
CHEMBL3334561 Inhibition = 15.9 % -
CHEMBL3334556 Inhibition = 34.7 % -
CHEMBL3334557 Inhibition = 61.0 % -
CHEMBL3334542 Inhibition = 36.9 % -
CHEMBL3334551 Inhibition = 50.1 % -
CHEMBL3334552 Inhibition = 18.8 % -