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Assay Detail

CHEMBL3369685

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Target occupancy of PDE10A in Sprague-Dawley rat striatum measured 1 hr post dosing and 10 mins post iv dosing of tracer by LC-MS/MS method
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Tissue Striatum
Confidence 1 — Organism
Curated By Autocuration

Target

Rattus norvegicus (CHEMBL376)
Type ORGANISM
Organism Rattus norvegicus

Publication

Discovery of clinical candidate 1-(4-(3-(4-(1H-benzo[d]imidazole-2-carbonyl)phenoxy)pyrazin-2-yl)piperidin-1-yl)ethanone (AMG 579), a potent, selective, and efficacious inhibitor of phosphodiesterase 10A (PDE10A).
Hu E, Chen N, Bourbeau MP, Harrington PE, Biswas K, Kunz RK, Andrews KL, Chmait S, Zhao X, Davis C, Ma J, Shi J, Lester-Zeiner D, Danao J, Able J, Cueva M, Talreja S, Kornecook T, Chen H, Porter A, Hungate R, Treanor J, Allen JR.
J Med Chem (2014) 57 : 6632 -6641
PubMed 25062128 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Occ 3 - -
EC50 3 6.36 6.93

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3319207 2 6.93
CHEMBL3319209 2 6.15
CHEMBL3319210 2 6.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3319207 EC50 = 117.0 nM 6.93
CHEMBL3319209 EC50 = 711.0 nM 6.15
CHEMBL3319210 EC50 = 965.0 nM 6.01
CHEMBL3319210 Occ - -
CHEMBL3319209 Occ - -
CHEMBL3319207 Occ - -