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Assay Detail

CHEMBL3370341

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human GST-thrombin-tagged MDM2 assessed as inhibition of interaction with human p53 after 1 hr by HTRF assay in presence of 15% human serum
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase Mdm2 (CHEMBL5023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization beyond AMG 232: discovery and SAR of sulfonamides on a piperidinone scaffold as potent inhibitors of the MDM2-p53 protein-protein interaction.
Wang Y, Zhu J, Liu JJ, Chen X, Mihalic J, Deignan J, Yu M, Sun D, Kayser F, McGee LR, Lo MC, Chen A, Zhou J, Ye Q, Huang X, Long AM, Yakowec P, Oliner JD, Olson SH, Medina JC.
Bioorg Med Chem Lett (2014) 24 : 3782 -3785
PubMed 25042256 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 9.75 10.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3318783 1 10.23
CHEMBL3318761 1 10.23
CHEMBL3318768 1 10.15
CHEMBL3318781 1 10.13
CHEMBL3318771 1 10.12
CHEMBL3318756 1 10.10
CHEMBL3318758 1 10.09
CHEMBL3318762 1 10.07
CHEMBL3318782 1 10.06
CHEMBL3318764 1 10.04
CHEMBL3318767 1 10.02
CHEMBL3318760 1 10.01
CHEMBL3318757 1 9.96
CHEMBL3318769 1 9.92
CHEMBL3318786 1 9.92
CHEMBL3318763 1 9.92
CHEMBL3318779 1 9.82
CHEMBL3318765 1 9.82
CHEMBL3318770 1 9.80
CHEMBL3318759 1 9.77
CHEMBL3318787 1 9.66
CHEMBL3318775 1 9.62
CHEMBL3318773 1 9.52
CHEMBL3318774 1 9.51
CHEMBL3318784 1 9.43
CHEMBL3318777 1 9.42
CHEMBL3318776 1 9.40
CHEMBL3318785 1 9.38
CHEMBL3318778 1 9.33
CHEMBL3318772 1 9.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3318783 IC50 = 0.059 nM 10.23
CHEMBL3318761 IC50 = 0.059 nM 10.23
CHEMBL3318768 IC50 = 0.071 nM 10.15
CHEMBL3318781 IC50 = 0.074 nM 10.13
CHEMBL3318771 IC50 = 0.075 nM 10.12
CHEMBL3318756 IC50 = 0.079 nM 10.10
CHEMBL3318758 IC50 = 0.081 nM 10.09
CHEMBL3318762 IC50 = 0.086 nM 10.07
CHEMBL3318782 IC50 = 0.088 nM 10.06
CHEMBL3318764 IC50 = 0.091 nM 10.04
CHEMBL3318767 IC50 = 0.096 nM 10.02
CHEMBL3318760 IC50 = 0.098 nM 10.01
CHEMBL3318757 IC50 = 0.11 nM 9.96
CHEMBL3318763 IC50 = 0.12 nM 9.92
CHEMBL3318786 IC50 = 0.12 nM 9.92
CHEMBL3318769 IC50 = 0.12 nM 9.92
CHEMBL3318779 IC50 = 0.15 nM 9.82
CHEMBL3318765 IC50 = 0.15 nM 9.82
CHEMBL3318770 IC50 = 0.16 nM 9.80
CHEMBL3318759 IC50 = 0.17 nM 9.77
CHEMBL3318787 IC50 = 0.22 nM 9.66
CHEMBL3318775 IC50 = 0.24 nM 9.62
CHEMBL3318773 IC50 = 0.3 nM 9.52
CHEMBL3318774 IC50 = 0.31 nM 9.51
CHEMBL3318784 IC50 = 0.37 nM 9.43
CHEMBL3318777 IC50 = 0.38 nM 9.42
CHEMBL3318776 IC50 = 0.4 nM 9.40
CHEMBL3318785 IC50 = 0.42 nM 9.38
CHEMBL3318778 IC50 = 0.47 nM 9.33
CHEMBL3318772 IC50 = 0.53 nM 9.28
CHEMBL3318766 IC50 = 0.64 nM 9.19
CHEMBL3318755 IC50 = 0.98 nM 9.01
CHEMBL3318780 IC50 = 1.1 nM 8.96