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Assay Detail

CHEMBL3370547

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine spleen cathepsin B using Z-Arg-Arg-AMC as substrate after 30 mins by fluorescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Tissue Spleen
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin B (CHEMBL2323)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Lower homologues of ahpatinin, aspartic protease inhibitors, from a marine Streptomyces sp.
Sun Y, Takada K, Nogi Y, Okada S, Matsunaga S.
J Nat Prod (2014) 77 : 1749 -1752
PubMed 24960234 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.72 5.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3314610 1 5.00
CHEMBL3314607 1 4.82
CHEMBL301728 ACETYLPEPSTATIN 1 4.77
CHEMBL3314608 1 4.60
CHEMBL3314609 1 4.58
CHEMBL3314611 1 4.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3314610 IC50 = 10000.0 nM 5.00
CHEMBL3314607 IC50 = 15000.0 nM 4.82
CHEMBL301728 ACETYLPEPSTATIN IC50 = 17000.0 nM 4.77
CHEMBL3314608 IC50 = 25000.0 nM 4.60
CHEMBL3314609 IC50 = 26000.0 nM 4.58
CHEMBL3314611 IC50 = 29000.0 nM 4.54