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Assay Detail

CHEMBL3370899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at glucocorticoid receptor in human HeLa cells assessed as NP-1 transactivation by luciferase reporter gene assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL2034)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of acylurea isosteres of 2-acylaminothiadiazole in the azaxanthene series of glucocorticoid receptor agonists.
Gong H, Yang M, Xiao Z, Doweyko AM, Cunningham M, Wang J, Habte S, Holloway D, Burke C, Shuster D, Gao L, Carman J, Somerville JE, Nadler SG, Salter-Cid L, Barrish JC, Weinstein DS.
Bioorg Med Chem Lett (2014) 24 : 3268 -3273
PubMed 24980053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 24 7.01 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3315066 1 8.89
CHEMBL384467 DEXAMETHASONE 4.0 1 8.35
CHEMBL3315064 1 8.28
CHEMBL3315061 1 8.26
CHEMBL3315062 1 7.97
CHEMBL3315063 1 7.96
CHEMBL3315052 1 7.85
CHEMBL3315051 1 7.57
CHEMBL3315065 1 7.31
CHEMBL3315070 1 7.23
CHEMBL3315049 1 7.01
CHEMBL3315069 1 6.97
CHEMBL3315067 1 6.95
CHEMBL3315068 1 6.84
CHEMBL3315054 1 6.75
CHEMBL1911270 1 6.62
CHEMBL3315058 1 6.62
CHEMBL3315057 1 6.52
CHEMBL3315055 1 6.23
CHEMBL3315059 1 6.17
CHEMBL3315053 1 5.98
CHEMBL3315050 1 5.98
CHEMBL3315056 1 5.05
CHEMBL3315060 1 4.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3315066 EC50 = 1.3 nM 8.89
CHEMBL384467 DEXAMETHASONE EC50 = 4.5 nM 8.35
CHEMBL3315064 EC50 = 5.3 nM 8.28
CHEMBL3315061 EC50 = 5.5 nM 8.26
CHEMBL3315062 EC50 = 10.8 nM 7.97
CHEMBL3315063 EC50 = 11.0 nM 7.96
CHEMBL3315052 EC50 = 14.0 nM 7.85
CHEMBL3315051 EC50 = 27.0 nM 7.57
CHEMBL3315065 EC50 = 49.3 nM 7.31
CHEMBL3315070 EC50 = 59.2 nM 7.23
CHEMBL3315049 EC50 = 98.5 nM 7.01
CHEMBL3315069 EC50 = 107.4 nM 6.97
CHEMBL3315067 EC50 = 112.6 nM 6.95
CHEMBL3315068 EC50 = 142.9 nM 6.84
CHEMBL3315054 EC50 = 177.0 nM 6.75
CHEMBL1911270 EC50 = 242.0 nM 6.62
CHEMBL3315058 EC50 = 241.0 nM 6.62
CHEMBL3315057 EC50 = 302.0 nM 6.52
CHEMBL3315055 EC50 = 586.0 nM 6.23
CHEMBL3315059 EC50 = 676.0 nM 6.17
CHEMBL3315053 EC50 = 1043.0 nM 5.98
CHEMBL3315050 EC50 = 1046.0 nM 5.98
CHEMBL3315056 EC50 = 8912.0 nM 5.05
CHEMBL3315060 EC50 = 10530.0 nM 4.98