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Assay Detail

CHEMBL3372282

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JNK3 in human SH-SY5Y cells assessed as reduction in c-Jun phosphorylation by Western blot assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 10 (CHEMBL2637)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of highly potent and isoform selective JNK3 inhibitors: SAR studies on aminopyrazole derivatives.
Zheng K, Iqbal S, Hernandez P, Park H, LoGrasso PV, Feng Y.
J Med Chem (2014) 57 : 10013 -10030
PubMed 25393557 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.80 6.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3355165 1 6.06
CHEMBL3355168 1 6.04
CHEMBL3355177 1 5.84
CHEMBL3355181 1 5.72
CHEMBL3355166 1 5.63
CHEMBL3355174 1 5.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3355165 IC50 = 866.0 nM 6.06
CHEMBL3355168 IC50 = 905.0 nM 6.04
CHEMBL3355177 IC50 = 1436.0 nM 5.84
CHEMBL3355181 IC50 = 1895.0 nM 5.72
CHEMBL3355166 IC50 = 2331.0 nM 5.63
CHEMBL3355174 IC50 = 3250.0 nM 5.49