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Assay Detail

CHEMBL3372768

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of lipopolysaccharide-induced nitric oxide production incubated for 24 hrs by Griess reagent based assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Anti-inflammatory ursane- and oleanane-type triterpenoids from Vitex negundo var. cannabifolia.
Li MM, Su XQ, Sun J, Gu YF, Huang Z, Zeng KW, Zhang Q, Zhao YF, Ferreira D, Zjawiony JK, Li J, Tu PF.
J Nat Prod (2014) 77 : 2248 -2254
PubMed 25245917 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.56 4.78

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL50 QUERCETIN 3.0 1 4.78
CHEMBL239077 TOMENTIC ACID 1 4.60
CHEMBL3342057 1 4.58
CHEMBL383749 1 4.56
CHEMBL3342053 1 4.47
CHEMBL3342058 1 4.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL50 QUERCETIN IC50 = 16600.0 nM 4.78
CHEMBL239077 TOMENTIC ACID IC50 = 24900.0 nM 4.60
CHEMBL3342057 IC50 = 26100.0 nM 4.58
CHEMBL383749 IC50 = 27700.0 nM 4.56
CHEMBL3342053 IC50 = 34000.0 nM 4.47
CHEMBL3342058 IC50 = 40500.0 nM 4.39