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Assay Detail

CHEMBL3374901

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant PDE4B1 activity assessed as residual cAMP concentration by HTRF assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4B (CHEMBL275)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Roflumilast analogs as soft PDE4 inhibitors.
Boland S, Alen J, Bourin A, Castermans K, Boumans N, Panitti L, Vanormelingen J, Leysen D, Defert O.
Bioorg Med Chem Lett (2014) 24 : 4594 -4597
PubMed 25149511 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.51 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 9.40
CHEMBL3327321 1 8.74
CHEMBL3327323 1 8.40
CHEMBL3327325 1 8.33
CHEMBL3327315 1 8.27
CHEMBL3327322 1 8.26
CHEMBL3327326 1 8.06
CHEMBL3327317 1 7.96
CHEMBL3327316 1 7.92
CHEMBL3327328 1 7.68
CHEMBL63 ROLIPRAM 2.0 1 7.28
CHEMBL3327327 1 7.06
CHEMBL3327324 1 6.75
CHEMBL3327318 1 6.13
CHEMBL3327329 1 6.04
CHEMBL3327319 1 5.77
CHEMBL3327320 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 0.4 nM 9.40
CHEMBL3327321 IC50 = 1.8 nM 8.74
CHEMBL3327323 IC50 = 4.0 nM 8.40
CHEMBL3327325 IC50 = 4.7 nM 8.33
CHEMBL3327315 IC50 = 5.4 nM 8.27
CHEMBL3327322 IC50 = 5.5 nM 8.26
CHEMBL3327326 IC50 = 8.8 nM 8.06
CHEMBL3327317 IC50 = 11.0 nM 7.96
CHEMBL3327316 IC50 = 12.0 nM 7.92
CHEMBL3327328 IC50 = 21.0 nM 7.68
CHEMBL63 ROLIPRAM IC50 = 52.0 nM 7.28
CHEMBL3327327 IC50 = 88.0 nM 7.06
CHEMBL3327324 IC50 = 180.0 nM 6.75
CHEMBL3327318 IC50 = 740.0 nM 6.13
CHEMBL3327329 IC50 = 910.0 nM 6.04
CHEMBL3327319 IC50 = 1700.0 nM 5.77
CHEMBL3327320 IC50 = 2700.0 nM 5.57