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Assay Detail

CHEMBL3375466

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BTK (unknown origin) using biotin-labeled peptide substrate incubated for 1 hr by TR-FRET based HTRF kinase assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and evaluation of novel 5-phenylpyridin-2(1H)-one derivatives as potent reversible Bruton's tyrosine kinase inhibitors.
Zhao X, Xin M, Huang W, Ren Y, Jin Q, Tang F, Jiang H, Wang Y, Yang J, Mo S, Xiang H.
Bioorg Med Chem (2015) 23 : 348 -364
PubMed 25515957 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.88 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3358397 1 8.05
CHEMBL2057918 1 7.89
CHEMBL3358396 1 7.80
CHEMBL3358395 1 7.70
CHEMBL3358398 1 7.55
CHEMBL3358399 1 7.43
CHEMBL3358408 1 6.69
CHEMBL3358401 1 6.59
CHEMBL3358407 1 6.44
CHEMBL3358402 1 6.37
CHEMBL3358406 1 6.30
CHEMBL3358400 1 6.25
CHEMBL3358405 1 6.09
CHEMBL3358409 1 6.06
CHEMBL3358403 1 5.99
CHEMBL3358412 1 -
CHEMBL3358404 1 -
CHEMBL3358410 1 -
CHEMBL3358411 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3358397 IC50 = 9.0 nM 8.05
CHEMBL2057918 IC50 = 13.0 nM 7.89
CHEMBL3358396 IC50 = 16.0 nM 7.80
CHEMBL3358395 IC50 = 20.0 nM 7.70
CHEMBL3358398 IC50 = 28.0 nM 7.55
CHEMBL3358399 IC50 = 37.0 nM 7.43
CHEMBL3358408 IC50 = 203.0 nM 6.69
CHEMBL3358401 IC50 = 255.0 nM 6.59
CHEMBL3358407 IC50 = 365.0 nM 6.44
CHEMBL3358402 IC50 = 431.0 nM 6.37
CHEMBL3358406 IC50 = 503.0 nM 6.30
CHEMBL3358400 IC50 = 564.0 nM 6.25
CHEMBL3358405 IC50 = 818.0 nM 6.09
CHEMBL3358409 IC50 = 875.0 nM 6.06
CHEMBL3358403 IC50 = 1030.0 nM 5.99
CHEMBL3358412 IC50 > 5000.0 nM -
CHEMBL3358404 IC50 > 5000.0 nM -
CHEMBL3358410 IC50 > 5000.0 nM -
CHEMBL3358411 IC50 > 5000.0 nM -