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Assay Detail

CHEMBL3376525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Pseudomonas aeruginosa pseudolysin using Abz-Ala-Gly-Leu-Ala-p-nitrobenzylamide as substrate after 15 mins
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Elastase (CHEMBL1075146)
Type SINGLE PROTEIN
Organism Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228)

Publication

Inhibition of pseudolysin and thermolysin by hydroxamate-based MMP inhibitors.
Adekoya OA, Sjøli S, Wuxiuer Y, Bilto I, Marques SM, Santos MA, Nuti E, Cercignani G, Rossello A, Winberg JO, Sylte I.
Eur J Med Chem (2015) 89 : 340 -348
PubMed 25462250 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 4.93 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL234737 1 6.40
CHEMBL3361134 1 5.17
CHEMBL234529 1 4.82
CHEMBL234530 1 4.80
CHEMBL3361129 1 4.70
CHEMBL234735 1 4.51
CHEMBL515980 1 4.09
CHEMBL3361130 1 -
CHEMBL3361131 1 -
CHEMBL515083 1 -
CHEMBL3361132 1 -
CHEMBL472899 1 -
CHEMBL3361133 1 -
CHEMBL234528 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL234737 IC50 = 400.0 nM 6.40
CHEMBL3361134 IC50 = 6700.0 nM 5.17
CHEMBL234529 IC50 = 15000.0 nM 4.82
CHEMBL234530 IC50 = 16000.0 nM 4.80
CHEMBL3361129 IC50 = 20000.0 nM 4.70
CHEMBL234735 IC50 = 31000.0 nM 4.51
CHEMBL515980 IC50 = 82000.0 nM 4.09
CHEMBL3361130 IC50 > 121000.0 nM -
CHEMBL3361131 IC50 > 200000.0 nM -
CHEMBL515083 IC50 = 169000.0 nM -
CHEMBL3361132 IC50 > 150000.0 nM -
CHEMBL472899 IC50 > 186000.0 nM -
CHEMBL3361133 IC50 > 330000.0 nM -
CHEMBL234528 IC50 > 282000.0 nM -