Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3383626

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human SIRT2 expressed in Escherichia coli BL21 (DE3) cells using fluorogenic 7-amino-4-methylcoumarin (AMC)-labeled peptide by fluorescence assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

NAD-dependent protein deacetylase sirtuin-2 (CHEMBL4462)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent and selective sirtuin 2 (SIRT2) inhibitors using a fragment-based approach.
Cui H, Kamal Z, Ai T, Xu Y, More SS, Wilson DJ, Chen L.
J Med Chem (2014) 57 : 8340 -8357
PubMed 25275824 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.84 7.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3343652 1 7.32
CHEMBL3343653 1 7.11
CHEMBL3343654 1 6.36
CHEMBL3343533 1 6.36
CHEMBL3343534 1 6.31
CHEMBL3343656 1 6.25
CHEMBL3343535 1 6.09
CHEMBL3343532 1 6.06
CHEMBL3343655 1 6.02
CHEMBL3343651 1 6.01
CHEMBL224864 1 5.81
CHEMBL420311 1 5.71
CHEMBL3343523 1 5.37
CHEMBL3343525 1 5.20
CHEMBL3343530 1 5.12
CHEMBL3343531 1 5.01
CHEMBL3343528 1 5.01
CHEMBL1140 NIACINAMIDE 4.0 1 4.98
CHEMBL3343526 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3343652 IC50 = 48.3 nM 7.32
CHEMBL3343653 IC50 = 77.4 nM 7.11
CHEMBL3343654 IC50 = 434.0 nM 6.36
CHEMBL3343533 IC50 = 436.0 nM 6.36
CHEMBL3343534 IC50 = 489.0 nM 6.31
CHEMBL3343656 IC50 = 558.0 nM 6.25
CHEMBL3343535 IC50 = 811.0 nM 6.09
CHEMBL3343532 IC50 = 869.0 nM 6.06
CHEMBL3343655 IC50 = 955.0 nM 6.02
CHEMBL3343651 IC50 = 978.0 nM 6.01
CHEMBL224864 IC50 = 1560.0 nM 5.81
CHEMBL420311 IC50 = 1940.0 nM 5.71
CHEMBL3343523 IC50 = 4300.0 nM 5.37
CHEMBL3343525 IC50 = 6290.0 nM 5.20
CHEMBL3343530 IC50 = 7570.0 nM 5.12
CHEMBL3343531 IC50 = 9800.0 nM 5.01
CHEMBL3343528 IC50 = 9700.0 nM 5.01
CHEMBL1140 NIACINAMIDE IC50 = 10500.0 nM 4.98
CHEMBL3343526 IC50 = 12100.0 nM 4.92