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Assay Detail

CHEMBL3385584

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of acetylcholinesterase (unknown origin) assessed as inhibition of acetylcholine hydrolysis after 30 mins by Ellmann's method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biotransformation of huperzine A by a fungal endophyte of Huperzia serrata furnished sesquiterpenoid-alkaloid hybrids.
Ying YM, Shan WG, Zhan ZJ.
J Nat Prod (2014) 77 : 2054 -2059
PubMed 25222040 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.67 9.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL395280 HUPERZINE A 2.0 1 9.27
CHEMBL3338389 1 7.22
CHEMBL3338388 1 6.96
CHEMBL3338386 1 6.00
CHEMBL3338387 1 5.66
CHEMBL3355594 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL395280 HUPERZINE A IC50 = 0.54 nM 9.27
CHEMBL3338389 IC50 = 60.0 nM 7.22
CHEMBL3338388 IC50 = 110.0 nM 6.96
CHEMBL3338386 IC50 = 990.0 nM 6.00
CHEMBL3338387 IC50 = 2170.0 nM 5.66
CHEMBL3355594 IC50 = 12110.0 nM 4.92