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Assay Detail

CHEMBL3386099

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Activity at mouse MC5R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 5 (CHEMBL4489)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

1,4-disubstituted-[1,2,3]triazolyl-containing analogues of MT-II: design, synthesis, conformational analysis, and biological activity.
Testa C, Scrima M, Grimaldi M, D'Ursi AM, Dirain ML, Lubin-Germain N, Singh A, Haskell-Luevano C, Chorev M, Rovero P, Papini AM.
J Med Chem (2014) 57 : 9424 -9434
PubMed 25347033 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 24 9.48 10.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3358545 1 10.38
CHEMBL3358549 1 10.35
CHEMBL3358541 1 10.29
CHEMBL3358552 1 10.15
CHEMBL3358539 1 10.07
CHEMBL3358540 1 9.89
CHEMBL3358546 1 9.89
CHEMBL3358535 1 9.89
CHEMBL3358551 1 9.82
CHEMBL3358550 1 9.82
CHEMBL3358536 1 9.74
CHEMBL430239 MELATONAN 1 9.72
CHEMBL3352878 1 9.64
CHEMBL3358544 1 9.47
CHEMBL3358547 1 8.75
CHEMBL3358548 1 8.73
CHEMBL3358537 1 8.72
CHEMBL3358543 1 8.71
CHEMBL3358533 1 8.49
CHEMBL3358538 1 8.30
CHEMBL3358534 1 8.27
CHEMBL441738 AFAMELANOTIDE 4.0 1 -
CHEMBL2372859 1 -
CHEMBL3358542 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3358545 EC50 = 0.042 nM 10.38
CHEMBL3358549 EC50 = 0.045 nM 10.35
CHEMBL3358541 EC50 = 0.051 nM 10.29
CHEMBL3358552 EC50 = 0.07 nM 10.15
CHEMBL3358539 EC50 = 0.086 nM 10.07
CHEMBL3358540 EC50 = 0.13 nM 9.89
CHEMBL3358546 EC50 = 0.13 nM 9.89
CHEMBL3358535 EC50 = 0.13 nM 9.89
CHEMBL3358551 EC50 = 0.15 nM 9.82
CHEMBL3358550 EC50 = 0.15 nM 9.82
CHEMBL3358536 EC50 = 0.18 nM 9.74
CHEMBL430239 MELATONAN EC50 = 0.19 nM 9.72
CHEMBL3352878 EC50 = 0.23 nM 9.64
CHEMBL3358544 EC50 = 0.34 nM 9.47
CHEMBL3358547 EC50 = 1.76 nM 8.75
CHEMBL3358548 EC50 = 1.88 nM 8.73
CHEMBL3358537 EC50 = 1.9 nM 8.72
CHEMBL3358543 EC50 = 1.93 nM 8.71
CHEMBL3358533 EC50 = 3.22 nM 8.49
CHEMBL3358538 EC50 = 5.04 nM 8.30
CHEMBL3358534 EC50 = 5.33 nM 8.27
CHEMBL441738 AFAMELANOTIDE EC50 - -
CHEMBL2372859 EC50 - -
CHEMBL3358542 EC50 - -