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Assay Detail

CHEMBL3386599

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at APJ receptor in HEK293 cells assessed as inhibition of forskolin-induced cAMP level incubated for 5 mins prior to forskolin challenge measured after 30 mins by TR-FRET assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL1628481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The design and implementation of a generic lipopeptide scanning platform to enable the identification of 'locally acting' agonists for the apelin receptor.
McKeown SC, Zecri FJ, Fortier E, Taggart A, Sviridenko L, Adams CM, McAllister KH, Pin SS.
Bioorg Med Chem Lett (2014) 24 : 4871 -4875
PubMed 25241924 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 7.80 10.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3184840 1 10.91
CHEMBL3338689 1 8.57
CHEMBL3338686 1 7.67
CHEMBL3338677 1 7.47
CHEMBL3338681 1 7.38
CHEMBL3338687 1 7.02
CHEMBL3338680 1 6.70
CHEMBL3338699 1 6.65
CHEMBL3338685 1 -
CHEMBL3338684 1 -
CHEMBL3338683 1 -
CHEMBL3338682 1 -
CHEMBL3338690 1 -
CHEMBL3338693 1 -
CHEMBL3338679 1 -
CHEMBL3338678 1 -
CHEMBL3338691 1 -
CHEMBL3338688 1 -
CHEMBL3338700 1 -
CHEMBL3338698 1 -
CHEMBL3337468 1 -
CHEMBL3338697 1 -
CHEMBL3338696 1 -
CHEMBL3338695 1 -
CHEMBL3338694 1 -
CHEMBL3338692 1 -
CHEMBL3338701 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3184840 EC50 = 0.0122 nM 10.91
CHEMBL3338689 EC50 = 2.7 nM 8.57
CHEMBL3338686 EC50 = 21.5 nM 7.67
CHEMBL3338677 EC50 = 33.7 nM 7.47
CHEMBL3338681 EC50 = 41.3 nM 7.38
CHEMBL3338687 EC50 = 95.7 nM 7.02
CHEMBL3338680 EC50 = 198.6 nM 6.70
CHEMBL3338699 EC50 = 225.5 nM 6.65
CHEMBL3338685 EC50 > 30000.0 nM -
CHEMBL3338684 EC50 > 30000.0 nM -
CHEMBL3338683 EC50 > 30000.0 nM -
CHEMBL3338682 EC50 > 30000.0 nM -
CHEMBL3338690 EC50 > 30000.0 nM -
CHEMBL3338693 EC50 > 30000.0 nM -
CHEMBL3338679 EC50 > 30000.0 nM -
CHEMBL3338678 EC50 > 30000.0 nM -
CHEMBL3338691 EC50 > 30000.0 nM -
CHEMBL3338688 EC50 > 30000.0 nM -
CHEMBL3338700 EC50 > 30000.0 nM -
CHEMBL3338698 EC50 > 30000.0 nM -
CHEMBL3337468 EC50 > 30000.0 nM -
CHEMBL3338697 EC50 > 30000.0 nM -
CHEMBL3338696 EC50 > 30000.0 nM -
CHEMBL3338695 EC50 > 30000.0 nM -
CHEMBL3338694 EC50 > 30000.0 nM -
CHEMBL3338692 EC50 > 30000.0 nM -
CHEMBL3338701 EC50 > 30000.0 nM -