Assay Detail
Binding
CHEMBL3387066
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of canine lung PDE5 using [3H]cGMP substrate by radiolabeled nucleotide method
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Canis lupus familiaris |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL2304402) ↗| Type | SINGLE PROTEIN |
| Organism | Canis lupus familiaris |
Publication
Design and synthesis of novel 5-(3,4,5-trimethoxybenzoyl)-4-aminopyrimidine derivatives as potent and selective phosphodiesterase 5 inhibitors: scaffold hopping using a pseudo-ring by intramolecular hydrogen bond formation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 8.87 | 10.28 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3338455 | — | — | 1 | 10.28 |
| CHEMBL3338445 | — | — | 1 | 9.89 |
| CHEMBL3338448 | — | — | 1 | 9.42 |
| CHEMBL3338444 | — | — | 1 | 9.18 |
| CHEMBL3338452 | — | — | 1 | 9.13 |
| CHEMBL3338453 | — | — | 1 | 8.82 |
| CHEMBL3338447 | — | — | 1 | 8.82 |
| CHEMBL3338450 | — | — | 1 | 8.77 |
| CHEMBL3338454 | — | — | 1 | 8.74 |
| CHEMBL3338443 | — | — | 1 | 8.70 |
| CHEMBL3338451 | — | — | 1 | 8.57 |
| CHEMBL3338449 | — | — | 1 | 8.47 |
| CHEMBL3338442 | — | — | 1 | 8.46 |
| CHEMBL3338446 | — | — | 1 | 8.30 |
| CHEMBL3338441 | — | — | 1 | 7.47 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3338455 | — | IC50 | = | 0.053 | nM | 10.28 |
| CHEMBL3338445 | — | IC50 | = | 0.13 | nM | 9.89 |
| CHEMBL3338448 | — | IC50 | = | 0.38 | nM | 9.42 |
| CHEMBL3338444 | — | IC50 | = | 0.66 | nM | 9.18 |
| CHEMBL3338452 | — | IC50 | = | 0.74 | nM | 9.13 |
| CHEMBL3338453 | — | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL3338447 | — | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL3338450 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL3338454 | — | IC50 | = | 1.8 | nM | 8.74 |
| CHEMBL3338443 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3338451 | — | IC50 | = | 2.7 | nM | 8.57 |
| CHEMBL3338449 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL3338442 | — | IC50 | = | 3.5 | nM | 8.46 |
| CHEMBL3338446 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3338441 | — | IC50 | = | 34.0 | nM | 7.47 |