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Assay Detail

CHEMBL3387261

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity against human thromboxane A2 receptor alpha expressed in QBI-HEK293A cells assessed as reduction in I-BOP-induced inositol monophosphate production incubated for 15 to 60 mins before I-BOP addition by HTRF assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Thromboxane A2 receptor (CHEMBL2069)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent, long-acting cyclopentane-1,3-Dione thromboxane (A2)-receptor antagonists.
Wang X, Liu L, Huang L, Herbst-Robinson K, Cornec AS, James MJ, Sugiyama S, Bassetto M, Brancale A, Trojanowski JQ, Lee VM, Smith AB, Brunden KR, Ballatore C.
ACS Med Chem Lett (2014) 5 : 1015 -1020
PubMed 25221659 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.70 10.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3354618 1 10.82
CHEMBL3354616 1 10.28
CHEMBL3354614 1 8.41
CHEMBL3354615 1 8.19
CHEMBL3335473 1 7.97
CHEMBL3354613 1 7.95
CHEMBL2107786 TERUTROBAN 3.0 1 7.79
CHEMBL3354619 1 7.54
CHEMBL3335482 1 7.23
CHEMBL65121 1 6.28
CHEMBL3335481 1 6.05
CHEMBL3335483 1 6.05
CHEMBL3354617 1 5.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3354618 IC50 = 0.015 nM 10.82
CHEMBL3354616 IC50 = 0.052 nM 10.28
CHEMBL3354614 IC50 = 3.9 nM 8.41
CHEMBL3354615 IC50 = 6.5 nM 8.19
CHEMBL3335473 IC50 = 10.7 nM 7.97
CHEMBL3354613 IC50 = 11.2 nM 7.95
CHEMBL2107786 TERUTROBAN IC50 = 16.4 nM 7.79
CHEMBL3354619 IC50 = 29.2 nM 7.54
CHEMBL3335482 IC50 = 59.0 nM 7.23
CHEMBL65121 IC50 = 523.0 nM 6.28
CHEMBL3335481 IC50 = 891.0 nM 6.05
CHEMBL3335483 IC50 = 892.0 nM 6.05
CHEMBL3354617 IC50 = 2760.0 nM 5.56