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Assay Detail

CHEMBL3388115

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ENT1 expressed in porcine PK15NTD cells by cell-based [3H]5-uridine uptake assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PK15NTD
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Equilibrative nucleoside transporter 1 (CHEMBL1997)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dilazep analogues for the study of equilibrative nucleoside transporters 1 and 2 (ENT1 and ENT2).
Playa H, Lewis TA, Ting A, Suh BC, Muñoz B, Matuza R, Passer BJ, Schreiber SL, Buolamwini JK.
Bioorg Med Chem Lett (2014) 24 : 5801 -5804
PubMed 25454272 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.54 8.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353069 1 8.55
CHEMBL3353072 1 8.49
CHEMBL3353074 1 8.34
CHEMBL126075 DILAZEP 2.0 1 7.76
CHEMBL3353071 1 7.75
CHEMBL3353073 1 7.51
CHEMBL3353070 1 7.18
CHEMBL3353077 1 7.03
CHEMBL3353076 1 6.66
CHEMBL3353075 1 6.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353069 IC50 = 2.8 nM 8.55
CHEMBL3353072 IC50 = 3.2 nM 8.49
CHEMBL3353074 IC50 = 4.6 nM 8.34
CHEMBL126075 DILAZEP IC50 = 17.5 nM 7.76
CHEMBL3353071 IC50 = 17.7 nM 7.75
CHEMBL3353073 IC50 = 31.0 nM 7.51
CHEMBL3353070 IC50 = 66.1 nM 7.18
CHEMBL3353077 IC50 = 93.9 nM 7.03
CHEMBL3353076 IC50 = 217.0 nM 6.66
CHEMBL3353075 IC50 = 803.0 nM 6.09