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Assay Detail

CHEMBL3389022

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type EGFR phosphorylation in human LoVo cells after 2 hrs by fluorescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type LoVo
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent and selective EGFR inhibitor (AZD9291) of both sensitizing and T790M resistance mutations that spares the wild type form of the receptor.
Finlay MR, Anderton M, Ashton S, Ballard P, Bethel PA, Box MR, Bradbury RH, Brown SJ, Butterworth S, Campbell A, Chorley C, Colclough N, Cross DA, Currie GS, Grist M, Hassall L, Hill GB, James D, James M, Kemmitt P, Klinowska T, Lamont G, Lamont SG, Martin N, McFarland HL, Mellor MJ, Orme JP, Perkins D, Perkins P, Richmond G, Smith P, Ward RA, Waring MJ, Whittaker D, Wells S, Wrigley GL.
J Med Chem (2014) 57 : 8249 -8267
PubMed 25271963 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 4.72 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2426279 1 5.92
CHEMBL3353408 1 4.96
CHEMBL2426277 1 4.92
CHEMBL3353411 1 4.48
CHEMBL3353406 1 4.34
CHEMBL3353407 1 4.24
CHEMBL3353409 1 4.15
CHEMBL3353410 OSIMERTINIB 4.0 1 -
CHEMBL3353395 1 -
CHEMBL3353396 1 -
CHEMBL3353397 1 -
CHEMBL3353398 1 -
CHEMBL3353399 1 -
CHEMBL3353400 1 -
CHEMBL2426288 1 -
CHEMBL3353402 1 -
CHEMBL3353403 1 -
CHEMBL3353404 1 -
CHEMBL3353405 1 -
CHEMBL3353412 1 -
CHEMBL3353401 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2426279 IC50 = 1200.0 nM 5.92
CHEMBL3353408 IC50 = 11000.0 nM 4.96
CHEMBL2426277 IC50 = 12000.0 nM 4.92
CHEMBL3353411 IC50 = 33000.0 nM 4.48
CHEMBL3353406 IC50 = 46000.0 nM 4.34
CHEMBL3353407 IC50 = 58000.0 nM 4.24
CHEMBL3353409 IC50 = 71000.0 nM 4.15
CHEMBL3353410 OSIMERTINIB IC50 = 480000.0 nM -
CHEMBL3353395 IC50 = 10000000.0 nM -
CHEMBL3353396 IC50 = 1600000.0 nM -
CHEMBL3353397 IC50 = 6060000.0 nM -
CHEMBL3353398 IC50 = 5600000.0 nM -
CHEMBL3353399 IC50 = 7500000.0 nM -
CHEMBL3353400 IC50 = 11000000.0 nM -
CHEMBL2426288 IC50 > 20000.0 nM -
CHEMBL3353402 IC50 = 9000000.0 nM -
CHEMBL3353403 IC50 = 357000.0 nM -
CHEMBL3353404 IC50 = 145000.0 nM -
CHEMBL3353405 IC50 = 938000.0 nM -
CHEMBL3353412 IC50 = 786000.0 nM -
CHEMBL3353401 IC50 = 10300000.0 nM -