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Assay Detail

CHEMBL3389758

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MRP1 (unknown origin) over-expressed in MDCK cells assessed as calcein accumulation incubated for 30 mins prior to Calcein-AM addition measured after 30 mins by fluorescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Multidrug resistance-associated protein 1 (CHEMBL3004)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAR studies on tetrahydroisoquinoline derivatives: the role of flexibility and bioisosterism to raise potency and selectivity toward P-glycoprotein.
Capparelli E, Zinzi L, Cantore M, Contino M, Perrone MG, Luurtsema G, Berardi F, Perrone R, Colabufo NA.
J Med Chem (2014) 57 : 9983 -9994
PubMed 25379609 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 5.06 5.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL260599 1 5.55
CHEMBL260505 1 5.03
CHEMBL3352978 1 5.00
CHEMBL3352989 1 4.64
CHEMBL3352975 1 -
CHEMBL3352974 1 -
CHEMBL3352977 1 -
CHEMBL3352979 1 -
CHEMBL3352980 1 -
CHEMBL3352981 1 -
CHEMBL3352982 1 -
CHEMBL3352983 1 -
CHEMBL3352976 1 -
CHEMBL3352985 1 -
CHEMBL3352986 1 -
CHEMBL3352987 1 -
CHEMBL3352973 1 -
CHEMBL3352988 1 -
CHEMBL259582 1 -
CHEMBL260506 1 -
CHEMBL3352984 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL260599 EC50 = 2800.0 nM 5.55
CHEMBL260505 EC50 = 9300.0 nM 5.03
CHEMBL3352978 EC50 = 10000.0 nM 5.00
CHEMBL3352989 EC50 = 23000.0 nM 4.64
CHEMBL3352975 EC50 - -
CHEMBL3352974 EC50 - -
CHEMBL3352977 EC50 - -
CHEMBL3352979 EC50 - -
CHEMBL3352980 EC50 - -
CHEMBL3352981 EC50 - -
CHEMBL3352982 EC50 - -
CHEMBL3352983 EC50 - -
CHEMBL3352976 EC50 - -
CHEMBL3352985 EC50 - -
CHEMBL3352986 EC50 - -
CHEMBL3352987 EC50 - -
CHEMBL3352973 EC50 - -
CHEMBL3352988 EC50 - -
CHEMBL259582 EC50 - -
CHEMBL260506 EC50 - -
CHEMBL3352984 EC50 - -