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Assay Detail

CHEMBL3389960

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli DNA gyrase using fluorescence-tagged DNA by fluorescenct polarization assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

DNA gyrase (CHEMBL2094139)
Type PROTEIN COMPLEX
Organism Escherichia coli

Publication

Novel DNA gyrase inhibiting spiropyrimidinetriones with a benzisoxazole scaffold: SAR and in vivo characterization.
Basarab GS, Brassil P, Doig P, Galullo V, Haimes HB, Kern G, Kutschke A, McNulty J, Schuck VJ, Stone G, Gowravaram M.
J Med Chem (2014) 57 : 9078 -9095
PubMed 25286019 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.89 9.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3357980 1 9.54
CHEMBL3357561 1 9.42
CHEMBL3357982 1 7.89
CHEMBL3357983 1 7.58
CHEMBL3357991 1 6.68
CHEMBL3357993 1 6.58
CHEMBL8 CIPROFLOXACIN 4.0 1 6.57
CHEMBL3357990 1 6.47
CHEMBL3357988 1 6.47
CHEMBL3357984 1 6.43
CHEMBL3357986 1 6.32
CHEMBL3357985 1 6.21
CHEMBL3357992 1 6.01
CHEMBL3357987 1 5.80
CHEMBL3357989 1 5.40
CHEMBL3357981 1 -
CHEMBL126 LINEZOLID 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3357980 IC50 = 0.29 nM 9.54
CHEMBL3357561 IC50 = 0.38 nM 9.42
CHEMBL3357982 IC50 = 13.0 nM 7.89
CHEMBL3357983 IC50 = 26.0 nM 7.58
CHEMBL3357991 IC50 = 210.0 nM 6.68
CHEMBL3357993 IC50 = 260.0 nM 6.58
CHEMBL8 CIPROFLOXACIN IC50 = 270.0 nM 6.57
CHEMBL3357990 IC50 = 340.0 nM 6.47
CHEMBL3357988 IC50 = 340.0 nM 6.47
CHEMBL3357984 IC50 = 370.0 nM 6.43
CHEMBL3357986 IC50 = 480.0 nM 6.32
CHEMBL3357985 IC50 = 610.0 nM 6.21
CHEMBL3357992 IC50 = 970.0 nM 6.01
CHEMBL3357987 IC50 = 1600.0 nM 5.80
CHEMBL3357989 IC50 = 4000.0 nM 5.40
CHEMBL3357981 IC50 > 83.0 nM -
CHEMBL126 LINEZOLID IC50 > 32000.0 nM -