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Assay Detail

CHEMBL3390699

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at PAR4 in PAR-4-AP-stimulated human platelets compound pretreated for 5 mins by fluorescent PAC1 integrin alpha2bb3 activation assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteinase-activated receptor 4 (CHEMBL4691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substituted indoles as selective protease activated receptor 4 (PAR-4) antagonists: Discovery and SAR of ML354.
Wen W, Young SE, Duvernay MT, Schulte ML, Nance KD, Melancon BJ, Engers J, Locuson CW, Wood MR, Daniels JS, Wu W, Lindsley CW, Hamm HE, Stauffer SR.
Bioorg Med Chem Lett (2014) 24 : 4708 -4713
PubMed 25176330 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.26 7.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3334887 1 7.18
CHEMBL125021 1 6.89
CHEMBL1609104 1 6.85
CHEMBL3334944 1 6.55
CHEMBL3334941 1 6.47
CHEMBL3334940 1 6.19
CHEMBL3334939 1 6.11
CHEMBL3334942 1 6.10
CHEMBL3334943 1 5.29
CHEMBL3334933 1 5.00
CHEMBL3334901 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3334887 IC50 = 66.0 nM 7.18
CHEMBL125021 IC50 = 130.0 nM 6.89
CHEMBL1609104 IC50 = 140.0 nM 6.85
CHEMBL3334944 IC50 = 279.0 nM 6.55
CHEMBL3334941 IC50 = 336.0 nM 6.47
CHEMBL3334940 IC50 = 646.0 nM 6.19
CHEMBL3334939 IC50 = 785.0 nM 6.11
CHEMBL3334942 IC50 = 793.0 nM 6.10
CHEMBL3334943 IC50 = 5130.0 nM 5.29
CHEMBL3334933 IC50 = 10000.0 nM 5.00
CHEMBL3334901 IC50 > 10000.0 nM -