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Assay Detail

CHEMBL3391289

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of telomerase extracted from HEK293 cells using 5'-bAATCCGTCGAGCAGAGTT as primer assessed as polymerization of telomeric repeats to the end of the primers after 2 hrs by Flash-Plate assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Telomerase reverse transcriptase (CHEMBL2916)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Natural products targeting telomere maintenance
Chen JL, Sperry J, Ip NY, Brimble MA
Medchemcomm (2011) 2 : 229 -245
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.40 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL222541 1 6.70
CHEMBL420982 1 5.52
CHEMBL221503 1 4.93
CHEMBL75267 7,8-DIHYDROXYFLAVONE 1 4.44
CHEMBL222211 1 -
CHEMBL301624 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL222541 IC50 = 200.0 nM 6.70
CHEMBL420982 IC50 = 3000.0 nM 5.52
CHEMBL221503 IC50 = 11700.0 nM 4.93
CHEMBL75267 7,8-DIHYDROXYFLAVONE IC50 = 36000.0 nM 4.44
CHEMBL222211 IC50 > 40000.0 nM -
CHEMBL301624 IC50 > 40000.0 nM -