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Assay Detail

CHEMBL3391295

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of telomerase extracted from human K562 cells preincubated for 10 mins followed by dNTPs and telomerase substrate primer TS-A addition measured after 30 mins by TRAP assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type K562
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Telomerase reverse transcriptase (CHEMBL2916)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Natural products targeting telomere maintenance
Chen JL, Sperry J, Ip NY, Brimble MA
Medchemcomm (2011) 2 : 229 -245
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.90 5.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8659 OLEIC ACID 2.0 1 5.07
CHEMBL3139878 1 4.82
CHEMBL1236642 VACCENIC ACID 1 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8659 OLEIC ACID IC50 = 8600.0 nM 5.07
CHEMBL3139878 IC50 = 15000.0 nM 4.82
CHEMBL1236642 VACCENIC ACID IC50 = 16000.0 nM 4.80