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Assay Detail

CHEMBL3391333

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human NOX2 transfected in COS 22 cells assessed as inhibition of lithium dodecyl sulphate-stimulated ROS generation preincubated for 15 mins followed by lithium dodecyl sulphate challenge by cell-free assay in presence of NADPH
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS 22
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome b-245 heavy chain (CHEMBL1287627)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Bridged tetrahydroisoquinolines as selective NADPH oxidase 2 (Nox2) inhibitors.
Cifuentes-Pagano E, Saha J, Csányi G, Ghouleh IA, Sahoo S, Rodríguez A, Wipf P, Pagano PJ, Skoda EM.
Medchemcomm (2013) 4 : 1085 -1092
PubMed 24466406 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.52 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3347551 1 7.52
CHEMBL3347550 1 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3347551 IC50 = 30.0 nM 7.52
CHEMBL3347550 IC50 = 30.0 nM 7.52