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Assay Detail

CHEMBL3395161

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DAMGO from mu opioid receptor in CD1 mouse whole brain minus cerebellum membranes by liquid scintillation counting
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL2858)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Methoxyflavones from Stachys glutinosa with binding affinity to opioid receptors: in silico, in vitro, and in vivo studies.
Ruiu S, Anzani N, Orrù A, Floris C, Caboni P, Alcaro S, Maccioni E, Distinto S, Cottiglia F.
J Nat Prod (2015) 78 : 69 -76
PubMed 25562563 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 5.58 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL556578 MORPHINE HYDROCHLORIDE 3.0 1 8.92
CHEMBL476121 XANTHOMICROL 1 6.08
CHEMBL73930 TANGERETIN 1 4.79
CHEMBL312790 SIDERITIFLAVONE 1 4.73
CHEMBL312750 EUPATILIN -1.0 1 4.55
CHEMBL3394775 1 4.39
CHEMBL3394774 1 -
CHEMBL226512 GARDENIN B 1 -
CHEMBL74272 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL556578 MORPHINE HYDROCHLORIDE Ki = 1.2 nM 8.92
CHEMBL476121 XANTHOMICROL Ki = 830.0 nM 6.08
CHEMBL73930 TANGERETIN Ki = 16300.0 nM 4.79
CHEMBL312790 SIDERITIFLAVONE Ki = 18500.0 nM 4.73
CHEMBL312750 EUPATILIN Ki = 28000.0 nM 4.55
CHEMBL3394775 Ki = 40500.0 nM 4.39
CHEMBL3394774 Ki > 50000.0 nM -
CHEMBL226512 GARDENIN B Ki > 50000.0 nM -
CHEMBL74272 Ki > 50000.0 nM -