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Assay Detail

CHEMBL3395327

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages.
Lee JW, Lee JH, Lee C, Jin Q, Lee D, Kim Y, Hong JT, Lee MK, Hwang BY.
Bioorg Med Chem Lett (2015) 25 : 960 -962
PubMed 25592708 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.56 4.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3393209 1 4.87
CHEMBL225304 PIMAGEDINE 2.0 1 4.73
CHEMBL334918 MAACKIAIN 1 4.57
CHEMBL3314594 1 4.55
CHEMBL3393208 1 4.48
CHEMBL513023 MEDIORESINOL 1 4.39
CHEMBL267963 (+)-PINORESINOL 1 4.35
CHEMBL44 GENISTEIN 2.0 1 -
CHEMBL241608 CALYCOSIN 1 -
CHEMBL294878 1 -
CHEMBL361362 SYRINGARESINOL 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3393209 IC50 = 13600.0 nM 4.87
CHEMBL225304 PIMAGEDINE IC50 = 18700.0 nM 4.73
CHEMBL334918 MAACKIAIN IC50 = 27000.0 nM 4.57
CHEMBL3314594 IC50 = 28100.0 nM 4.55
CHEMBL3393208 IC50 = 33000.0 nM 4.48
CHEMBL513023 MEDIORESINOL IC50 = 41000.0 nM 4.39
CHEMBL267963 (+)-PINORESINOL IC50 = 45100.0 nM 4.35
CHEMBL44 GENISTEIN IC50 > 50000.0 nM -
CHEMBL241608 CALYCOSIN IC50 > 50000.0 nM -
CHEMBL294878 IC50 > 50000.0 nM -
CHEMBL361362 SYRINGARESINOL IC50 > 50000.0 nM -