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Assay Detail

CHEMBL3396875

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [20-3H] phorbol 12, 13-dibutylate from recombinant PKCalpha (unknown origin) in presence of 100 ug/ml 100% phosphatidylserine
22
Total Activities
22
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C alpha type (CHEMBL299)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of protein kinase C epsilon selective diacylglycerol lactones (DAG-lactones).
Ann J, Yoon S, Baek J, Kim DH, Lewin NE, Hill CS, Blumberg PM, Lee J.
Eur J Med Chem (2015) 90 : 332 -341
PubMed 25437619 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.92 8.56
Kd 1 9.55 9.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL27768 1 9.55
CHEMBL3393748 1 8.56
CHEMBL3393741 1 8.36
CHEMBL230048 1 8.32
CHEMBL3393654 1 8.15
CHEMBL3393753 1 8.14
CHEMBL137386 1 8.08
CHEMBL3393754 1 8.08
CHEMBL3393752 1 8.08
CHEMBL3393750 1 8.06
CHEMBL3393751 1 8.01
CHEMBL3393743 1 7.99
CHEMBL3393749 1 7.90
CHEMBL3393745 1 7.88
CHEMBL3393742 1 7.81
CHEMBL3393744 1 7.78
CHEMBL3393758 1 7.61
CHEMBL3393756 1 7.57
CHEMBL3393747 1 7.51
CHEMBL3393757 1 7.50
CHEMBL3393755 1 7.48
CHEMBL3393746 1 7.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL27768 Kd = 0.28 nM 9.55
CHEMBL3393748 Ki = 2.78 nM 8.56
CHEMBL3393741 Ki = 4.33 nM 8.36
CHEMBL230048 Ki = 4.74 nM 8.32
CHEMBL3393654 Ki = 7.05 nM 8.15
CHEMBL3393753 Ki = 7.2 nM 8.14
CHEMBL137386 Ki = 8.3 nM 8.08
CHEMBL3393754 Ki = 8.26 nM 8.08
CHEMBL3393752 Ki = 8.31 nM 8.08
CHEMBL3393750 Ki = 8.7 nM 8.06
CHEMBL3393751 Ki = 9.8 nM 8.01
CHEMBL3393743 Ki = 10.2 nM 7.99
CHEMBL3393749 Ki = 12.6 nM 7.90
CHEMBL3393745 Ki = 13.2 nM 7.88
CHEMBL3393742 Ki = 15.4 nM 7.81
CHEMBL3393744 Ki = 16.5 nM 7.78
CHEMBL3393758 Ki = 24.5 nM 7.61
CHEMBL3393756 Ki = 26.7 nM 7.57
CHEMBL3393747 Ki = 30.8 nM 7.51
CHEMBL3393757 Ki = 31.6 nM 7.50
CHEMBL3393755 Ki = 33.1 nM 7.48
CHEMBL3393746 Ki = 43.9 nM 7.36