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Assay Detail

CHEMBL3399458

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse GAT4 expressed in HEK293 cells by [3H]GABA uptake assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

GABA transporter 4 (CHEMBL3699)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Design, synthesis and SAR studies of GABA uptake inhibitors derived from 2-substituted pyrrolidine-2-yl-acetic acids.
Steffan T, Renukappa-Gutke T, Höfner G, Wanner KT.
Bioorg Med Chem (2015) 23 : 1284 -1306
PubMed 25698617 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.88 5.81

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL75035 1 5.81
CHEMBL3398502 1 5.24
CHEMBL3398503 1 5.18
CHEMBL3398494 1 4.85
CHEMBL3398504 1 4.76
CHEMBL3398496 1 4.67
CHEMBL3398506 1 4.50
CHEMBL3398507 1 4.00
CHEMBL1163348 1 -
CHEMBL1164855 1 -
CHEMBL3398490 1 -
CHEMBL3398497 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL75035 IC50 = 1548.82 nM 5.81
CHEMBL3398502 IC50 = 5754.4 nM 5.24
CHEMBL3398503 IC50 = 6606.93 nM 5.18
CHEMBL3398494 IC50 = 14125.38 nM 4.85
CHEMBL3398504 IC50 = 17378.01 nM 4.76
CHEMBL3398496 IC50 = 21379.62 nM 4.67
CHEMBL3398506 IC50 = 31622.78 nM 4.50
CHEMBL3398507 IC50 = 100000.0 nM 4.00
CHEMBL1163348 IC50 = 1122018.45 nM -
CHEMBL1164855 IC50 = 173780.08 nM -
CHEMBL3398490 IC50 = 338844.16 nM -
CHEMBL3398497 IC50 = 371535.23 nM -