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Assay Detail

CHEMBL3399809

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant mouse neutrophil elastase using methoxysuccinyl-Ala-Ala-Pro-Val-pnitroanilide as substrate preincubated for 15 mins before substrate addition measured after 90 mins by spectrophotometry
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neutrophil elastase (CHEMBL5156)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and pharmacological characterization of 2-aminobenzaldehyde oxime analogs as dual inhibitors of neutrophil elastase and proteinase 3.
Hwang TL, Wang WH, Wang TY, Yu HP, Hsieh PW.
Bioorg Med Chem (2015) 23 : 1123 -1134
PubMed 25650311 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.78 6.84

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL76688 SIVELESTAT 3.0 1 6.84
CHEMBL3398148 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL76688 SIVELESTAT IC50 = 145.39 nM 6.84
CHEMBL3398148 IC50 = 190.75 nM 6.72