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Assay Detail

CHEMBL3404057

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at rat NTS2 overexpressed in CHO-k1 cells assessed as calcium mobilization by FLIPR assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neurotensin receptor type 2 (CHEMBL5106)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

The amide linker in nonpeptide neurotensin receptor ligands plays a key role in calcium signaling at the neurotensin receptor type 2.
Thomas JB, Giddings AM, Olepu S, Wiethe RW, Warner KR, Sarret P, Longpre JM, Runyon SP, Gilmour BP.
Bioorg Med Chem Lett (2015) 25 : 2060 -2064
PubMed 25881832 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.64 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3403507 1 7.26
CHEMBL3403508 1 6.02
CHEMBL3403504 1 -
CHEMBL3403505 1 -
CHEMBL3403506 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3403507 EC50 = 55.0 nM 7.26
CHEMBL3403508 EC50 = 951.0 nM 6.02
CHEMBL3403504 EC50 - -
CHEMBL3403505 EC50 - -
CHEMBL3403506 EC50 - -