Assay Detail
Binding
CHEMBL3404294
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant N-terminal His-tagged HGFA (unknown origin) expressed in baculovirus-infected Sf9 cells incubated for 30 mins prior to cromogenic substrate addition by spectrophotometry
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Hepatocyte growth factor activator (CHEMBL3351190) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-based discovery of small molecule hepsin and HGFA protease inhibitors: Evaluation of potency and selectivity derived from distinct binding pockets.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 5.85 | 6.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL273264 | NAFAMOSTAT | 3.0 | 1 | 6.82 |
| CHEMBL3403524 | — | — | 1 | 6.52 |
| CHEMBL3403525 | — | — | 1 | 6.22 |
| CHEMBL550687 | — | — | 1 | 6.09 |
| CHEMBL1195245 | — | — | 1 | 5.96 |
| CHEMBL3403523 | — | — | 1 | 5.52 |
| CHEMBL3403522 | — | — | 1 | 5.17 |
| CHEMBL590799 | CAMOSTAT | 3.0 | 1 | 4.50 |
| CHEMBL579653 | — | — | 1 | - |
| CHEMBL535214 | — | — | 1 | - |
| CHEMBL3403527 | — | — | 1 | - |
| CHEMBL3403526 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL273264 | NAFAMOSTAT | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL3403524 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL3403525 | — | IC50 | = | 600.0 | nM | 6.22 |
| CHEMBL550687 | — | IC50 | = | 820.0 | nM | 6.09 |
| CHEMBL1195245 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL3403523 | — | IC50 | = | 3000.0 | nM | 5.52 |
| CHEMBL3403522 | — | IC50 | = | 6800.0 | nM | 5.17 |
| CHEMBL590799 | CAMOSTAT | IC50 | = | 32000.0 | nM | 4.50 |
| CHEMBL579653 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL535214 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL3403527 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL3403526 | — | IC50 | > | 50000.0 | nM | - |