Assay Detail
Binding
CHEMBL3404295
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant N-terminal His-tagged HGFA (unknown origin) expressed in baculovirus-infected Sf9 cells using Boc-QLR-AMC as substrate incubated for 30 mins prior to substrate addition by fluorescence assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Hepatocyte growth factor activator (CHEMBL3351190) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-based discovery of small molecule hepsin and HGFA protease inhibitors: Evaluation of potency and selectivity derived from distinct binding pockets.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 16 | 5.15 | 7.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL273264 | NAFAMOSTAT | 3.0 | 1 | 7.60 |
| CHEMBL379186 | — | — | 1 | 5.26 |
| CHEMBL3403521 | — | — | 1 | 5.15 |
| CHEMBL3403528 | — | — | 1 | 5.00 |
| CHEMBL3403529 | — | — | 1 | 4.85 |
| CHEMBL3403531 | — | — | 1 | 4.79 |
| CHEMBL379586 | — | — | 1 | 4.77 |
| CHEMBL356792 | — | — | 1 | 4.74 |
| CHEMBL3403532 | — | — | 1 | 4.70 |
| CHEMBL3402440 | — | — | 1 | 4.65 |
| CHEMBL3402437 | — | — | 1 | - |
| CHEMBL3400827 | — | — | 1 | - |
| CHEMBL3403533 | — | — | 1 | - |
| CHEMBL3403530 | — | — | 1 | - |
| CHEMBL3402439 | — | — | 1 | - |
| CHEMBL3402438 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL273264 | NAFAMOSTAT | Ki | = | 25.0 | nM | 7.60 |
| CHEMBL379186 | — | Ki | = | 5500.0 | nM | 5.26 |
| CHEMBL3403521 | — | Ki | = | 7100.0 | nM | 5.15 |
| CHEMBL3403528 | — | Ki | = | 10100.0 | nM | 5.00 |
| CHEMBL3403529 | — | Ki | = | 14000.0 | nM | 4.85 |
| CHEMBL3403531 | — | Ki | = | 16200.0 | nM | 4.79 |
| CHEMBL379586 | — | Ki | = | 17000.0 | nM | 4.77 |
| CHEMBL356792 | — | Ki | = | 18200.0 | nM | 4.74 |
| CHEMBL3403532 | — | Ki | = | 20000.0 | nM | 4.70 |
| CHEMBL3402440 | — | Ki | = | 22200.0 | nM | 4.65 |
| CHEMBL3402437 | — | Ki | > | 50000.0 | nM | - |
| CHEMBL3400827 | — | Ki | > | 50000.0 | nM | - |
| CHEMBL3403533 | — | Ki | > | 50000.0 | nM | - |
| CHEMBL3403530 | — | Ki | > | 50000.0 | nM | - |
| CHEMBL3402439 | — | Ki | > | 50000.0 | nM | - |
| CHEMBL3402438 | — | Ki | > | 50000.0 | nM | - |