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Assay Detail

CHEMBL3404758

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CysLT1 receptor expressed in CHO cells assessed as inhibition of LTD4-inudced intracellular calcium influx preincubated for 30 mins before LTD4 addition by Fura2-AM assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cysteinyl leukotriene receptor 1 (CHEMBL1798)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent, orally available dual CysLT₁ and CysLT₂ antagonist with dicarboxylic acid.
Itadani S, Takahashi S, Ima M, Sekiguchi T, Aratani Y, Egashira H, Matsumura N, Inoue A, Yonetomi Y, Fujita M, Nakayama Y, Takeuchi J.
Bioorg Med Chem (2015) 23 : 2079 -2097
PubMed 25800431 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.36 9.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3403186 1 9.23
CHEMBL3401689 1 9.10
CHEMBL3342955 1 7.96
CHEMBL3403190 1 7.89
CHEMBL3401690 1 7.66
CHEMBL3403187 1 7.62
CHEMBL3403185 1 7.46
CHEMBL3401686 1 7.24
CHEMBL3401687 1 7.16
CHEMBL3401691 1 7.16
CHEMBL3401688 1 7.09
CHEMBL3403188 1 6.60
CHEMBL3403189 1 6.31
CHEMBL3403184 1 6.13
CHEMBL3401692 1 5.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3403186 IC50 = 0.59 nM 9.23
CHEMBL3401689 IC50 = 0.79 nM 9.10
CHEMBL3342955 IC50 = 11.0 nM 7.96
CHEMBL3403190 IC50 = 13.0 nM 7.89
CHEMBL3401690 IC50 = 22.0 nM 7.66
CHEMBL3403187 IC50 = 24.0 nM 7.62
CHEMBL3403185 IC50 = 35.0 nM 7.46
CHEMBL3401686 IC50 = 58.0 nM 7.24
CHEMBL3401687 IC50 = 70.0 nM 7.16
CHEMBL3401691 IC50 = 70.0 nM 7.16
CHEMBL3401688 IC50 = 82.0 nM 7.09
CHEMBL3403188 IC50 = 250.0 nM 6.60
CHEMBL3403189 IC50 = 490.0 nM 6.31
CHEMBL3403184 IC50 = 740.0 nM 6.13
CHEMBL3401692 IC50 = 1900.0 nM 5.72