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Assay Detail

CHEMBL3405102

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of TNFalpha production in mouse RAW264.7 cells incubated for 6 hrs by sandwich immunoassay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

2'-Hydroxy flavanone derivatives as an inhibitors of pro-inflammatory mediators: Experimental and molecular docking studies.
Patel NK, Bairwa K, Gangwal R, Jaiswal G, Jachak SM, Sangamwar AT, Bhutani KK.
Bioorg Med Chem Lett (2015) 25 : 1952 -1955
PubMed 25824662 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.11 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL384467 DEXAMETHASONE 4.0 1 7.40
CHEMBL140 CURCUMIN 3.0 1 5.47
CHEMBL3401941 1 4.75
CHEMBL3401939 1 4.70
CHEMBL3401942 1 4.70
CHEMBL3401940 1 4.62
CHEMBL75897 2'-HYDROXY-FLAVANONE 1 4.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL384467 DEXAMETHASONE IC50 = 40.0 nM 7.40
CHEMBL140 CURCUMIN IC50 = 3400.0 nM 5.47
CHEMBL3401941 IC50 = 17700.0 nM 4.75
CHEMBL3401939 IC50 = 20100.0 nM 4.70
CHEMBL3401942 IC50 = 19800.0 nM 4.70
CHEMBL3401940 IC50 = 23700.0 nM 4.62
CHEMBL75897 2'-HYDROXY-FLAVANONE IC50 = 71300.0 nM 4.15