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Assay Detail

CHEMBL3406979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NMS from human M2 receptor expressed in CHOK1 cell membranes after 4 hrs by scintillation counting method
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel quaternary ammonium derivatives of (3R)-quinuclidinyl amides as potent and long acting muscarinic antagonists.
Prat M, Buil MA, Fernández MD, Tort L, Monleón JM, Casals G, Ferrer M, Castro J, Gavaldà A, Miralpeix M, Ramos I, Vilella D, Huerta JM, Espinosa S, Hernández B, Segarra V, Córdoba M.
Bioorg Med Chem Lett (2015) 25 : 1736 -1741
PubMed 25800115 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.53 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3545181 TIOTROPIUM BROMIDE 4.0 1 9.70
CHEMBL3401649 1 9.22
CHEMBL3401648 1 9.22
CHEMBL3401650 1 9.05
CHEMBL3401641 1 8.77
CHEMBL1908368 1 8.70
CHEMBL3401643 1 8.38
CHEMBL3401651 1 8.32
CHEMBL3401654 1 7.53
CHEMBL3401640 1 7.46
CHEMBL3401652 1 7.04
CHEMBL3401645 1 7.00
CHEMBL3401655 1 6.87
CHEMBL3401656 1 6.85
CHEMBL3401642 1 6.64
CHEMBL3401647 1 6.54
CHEMBL3401646 1 6.46
CHEMBL3401653 1 6.09
CHEMBL3401644 2 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3545181 TIOTROPIUM BROMIDE IC50 = 0.2 nM 9.70
CHEMBL3401649 IC50 = 0.6 nM 9.22
CHEMBL3401648 IC50 = 0.6 nM 9.22
CHEMBL3401650 IC50 = 0.9 nM 9.05
CHEMBL3401641 IC50 = 1.7 nM 8.77
CHEMBL1908368 IC50 = 2.0 nM 8.70
CHEMBL3401643 IC50 = 4.2 nM 8.38
CHEMBL3401651 IC50 = 4.8 nM 8.32
CHEMBL3401654 IC50 = 29.8 nM 7.53
CHEMBL3401640 IC50 = 35.1 nM 7.46
CHEMBL3401652 IC50 = 91.5 nM 7.04
CHEMBL3401645 IC50 = 101.1 nM 7.00
CHEMBL3401655 IC50 = 136.6 nM 6.87
CHEMBL3401656 IC50 = 140.2 nM 6.85
CHEMBL3401642 IC50 = 229.6 nM 6.64
CHEMBL3401647 IC50 = 285.9 nM 6.54
CHEMBL3401646 IC50 = 347.6 nM 6.46
CHEMBL3401653 IC50 = 804.2 nM 6.09
CHEMBL3401644 IC50 = 2531.4 nM 5.60
CHEMBL3401644 IC50 = 6455.0 nM 5.19