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Assay Detail

CHEMBL3411683

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive binding to PLK4 (unknown origin) by double reciprocal plot analysis in presence of ATP
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PLK4 (CHEMBL3788)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of Polo-like kinase 4 inhibitors: identification of (1R,2S).2-(3-((E).4-(((cis).2,6-dimethylmorpholino)methyl)styryl). 1H.indazol-6-yl)-5'-methoxyspiro[cyclopropane-1,3'-indolin]-2'-one (CFI-400945) as a potent, orally active antitumor agent.
Sampson PB, Liu Y, Forrest B, Cumming G, Li SW, Patel NK, Edwards L, Laufer R, Feher M, Ban F, Awrey DE, Mao G, Plotnikova O, Hodgson R, Beletskaya I, Mason JM, Luo X, Nadeem V, Wei X, Kiarash R, Madeira B, Huang P, Mak TW, Pan G, Pauls HW.
J Med Chem (2015) 58 : 147 -169
PubMed 25723005 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 9.58 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3408945 1 10.00
CHEMBL3408947 OCIFISERTIB 2.0 1 9.59
CHEMBL3408946 1 9.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3408945 Ki = 0.1 nM 10.00
CHEMBL3408947 OCIFISERTIB Ki = 0.26 nM 9.59
CHEMBL3408946 Ki = 0.73 nM 9.14