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Assay Detail

CHEMBL3412166

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal His-tagged PERK expressed in Escherichia coli using AviTag C-terminal, N-terminal His-tagged eIF2alpha (3 to 315) as substrate by LanthaScreen TR-FRET assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of 1H-pyrazol-3(2H)-ones as potent and selective inhibitors of protein kinase R-like endoplasmic reticulum kinase (PERK).
Smith AL, Andrews KL, Beckmann H, Bellon SF, Beltran PJ, Booker S, Chen H, Chung YA, D'Angelo ND, Dao J, Dellamaggiore KR, Jaeckel P, Kendall R, Labitzke K, Long AM, Materna-Reichelt S, Mitchell P, Norman MH, Powers D, Rose M, Shaffer PL, Wu MM, Lipford JR.
J Med Chem (2015) 58 : 1426 -1441
PubMed 25587754 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 8.14 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3407849 1 9.00
CHEMBL3407846 1 9.00
CHEMBL3407863 1 8.70
CHEMBL3407504 1 8.52
CHEMBL3407850 1 8.52
CHEMBL3407848 1 8.40
CHEMBL3407856 1 8.40
CHEMBL3407852 1 8.40
CHEMBL3407854 1 8.30
CHEMBL3407851 1 8.30
CHEMBL3407859 1 8.22
CHEMBL3407853 1 8.22
CHEMBL3407862 1 8.22
CHEMBL3407857 1 8.05
CHEMBL3407860 1 7.96
CHEMBL3407858 1 7.96
CHEMBL3407847 1 7.39
CHEMBL3407864 1 7.38
CHEMBL3407861 1 7.28
CHEMBL3407855 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3407849 IC50 = 1.0 nM 9.00
CHEMBL3407846 IC50 = 1.0 nM 9.00
CHEMBL3407863 IC50 = 2.0 nM 8.70
CHEMBL3407504 IC50 = 3.0 nM 8.52
CHEMBL3407850 IC50 = 3.0 nM 8.52
CHEMBL3407848 IC50 = 4.0 nM 8.40
CHEMBL3407856 IC50 = 4.0 nM 8.40
CHEMBL3407852 IC50 = 4.0 nM 8.40
CHEMBL3407854 IC50 = 5.0 nM 8.30
CHEMBL3407851 IC50 = 5.0 nM 8.30
CHEMBL3407859 IC50 = 6.0 nM 8.22
CHEMBL3407853 IC50 = 6.0 nM 8.22
CHEMBL3407862 IC50 = 6.0 nM 8.22
CHEMBL3407857 IC50 = 9.0 nM 8.05
CHEMBL3407860 IC50 = 11.0 nM 7.96
CHEMBL3407858 IC50 = 11.0 nM 7.96
CHEMBL3407847 IC50 = 41.0 nM 7.39
CHEMBL3407864 IC50 = 42.0 nM 7.38
CHEMBL3407861 IC50 = 53.0 nM 7.28
CHEMBL3407855 IC50 = 240.0 nM 6.62