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Assay Detail

CHEMBL3412926

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant human neuronal NOS overexpressed in Escherichia coli assessed as production of nitric oxide from L-arginine measured for 5 mins by oxyhemoglobin NO capture assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 1 (CHEMBL3568)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel 2,4-disubstituted pyrimidines as potent, selective, and cell-permeable inhibitors of neuronal nitric oxide synthase.
Mukherjee P, Li H, Sevrioukova I, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB.
J Med Chem (2015) 58 : 1067 -1088
PubMed 25489882 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 20 6.64 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL480419 1 7.16
CHEMBL3407814 1 6.90
CHEMBL3407811 1 6.90
CHEMBL3407815 1 6.86
CHEMBL3407822 1 6.86
CHEMBL3407810 1 6.71
CHEMBL3407821 1 6.52
CHEMBL3407812 1 6.45
CHEMBL3407813 1 6.45
CHEMBL3407809 1 6.12
CHEMBL3407823 1 6.06
CHEMBL3407808 1 -
CHEMBL3407805 1 -
CHEMBL3407816 1 -
CHEMBL3407817 1 -
CHEMBL3407818 1 -
CHEMBL3407819 1 -
CHEMBL3407820 1 -
CHEMBL3407807 1 -
CHEMBL3407806 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL480419 Ki = 70.0 nM 7.16
CHEMBL3407814 Ki = 125.0 nM 6.90
CHEMBL3407811 Ki = 125.0 nM 6.90
CHEMBL3407815 Ki = 138.0 nM 6.86
CHEMBL3407822 Ki = 137.0 nM 6.86
CHEMBL3407810 Ki = 193.0 nM 6.71
CHEMBL3407821 Ki = 303.0 nM 6.52
CHEMBL3407812 Ki = 359.0 nM 6.45
CHEMBL3407813 Ki = 358.0 nM 6.45
CHEMBL3407809 Ki = 758.0 nM 6.12
CHEMBL3407823 Ki = 873.0 nM 6.06
CHEMBL3407808 Ki - -
CHEMBL3407805 Ki - -
CHEMBL3407816 Ki - -
CHEMBL3407817 Ki - -
CHEMBL3407818 Ki - -
CHEMBL3407819 Ki - -
CHEMBL3407820 Ki - -
CHEMBL3407807 Ki - -
CHEMBL3407806 Ki - -