Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3419557

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Vps75-stimulated recombinant Saccharomyces cerevisiae histone acetyltransferase Rtt109 using [3H]-acetyl-CoA assessed as acetate incorporation after 30 mins by liquid scintillation counting
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Saccharomyces cerevisiae
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Histone acetyltransferase RTT109 (CHEMBL3414417)
Type SINGLE PROTEIN
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Publication

PAINS in the assay: chemical mechanisms of assay interference and promiscuous enzymatic inhibition observed during a sulfhydryl-scavenging HTS.
Dahlin JL, Nissink JW, Strasser JM, Francis S, Higgins L, Zhou H, Zhang Z, Walters MA.
J Med Chem (2015) 58 : 2091 -2113
PubMed 25634295 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.81 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1331211 1 6.89
CHEMBL2031566 1 6.82
CHEMBL3416552 1 6.58
CHEMBL3416536 1 6.28
CHEMBL1385343 1 6.19
CHEMBL2030281 1 6.17
CHEMBL3416321 1 6.11
CHEMBL3416335 1 6.09
CHEMBL3416526 1 5.92
CHEMBL3416537 1 5.80
CHEMBL3235887 1 5.77
CHEMBL1600114 1 5.77
CHEMBL3416316 1 5.47
CHEMBL502489 GARCINOL 1 5.46
CHEMBL3416525 1 5.41
CHEMBL3416317 1 5.41
CHEMBL3235890 1 5.37
CHEMBL3416315 1 5.34
CHEMBL1602759 1 5.21
CHEMBL3416528 1 5.07
CHEMBL3416307 1 4.82
CHEMBL106 FLUCONAZOLE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1331211 IC50 = 130.0 nM 6.89
CHEMBL2031566 IC50 = 150.0 nM 6.82
CHEMBL3416552 IC50 = 260.0 nM 6.58
CHEMBL3416536 IC50 = 520.0 nM 6.28
CHEMBL1385343 IC50 = 640.0 nM 6.19
CHEMBL2030281 IC50 = 670.0 nM 6.17
CHEMBL3416321 IC50 = 770.0 nM 6.11
CHEMBL3416335 IC50 = 810.0 nM 6.09
CHEMBL3416526 IC50 = 1200.0 nM 5.92
CHEMBL3416537 IC50 = 1600.0 nM 5.80
CHEMBL3235887 IC50 = 1700.0 nM 5.77
CHEMBL1600114 IC50 = 1700.0 nM 5.77
CHEMBL3416316 IC50 = 3400.0 nM 5.47
CHEMBL502489 GARCINOL IC50 = 3500.0 nM 5.46
CHEMBL3416525 IC50 = 3900.0 nM 5.41
CHEMBL3416317 IC50 = 3900.0 nM 5.41
CHEMBL3235890 IC50 = 4300.0 nM 5.37
CHEMBL3416315 IC50 = 4600.0 nM 5.34
CHEMBL1602759 IC50 = 6100.0 nM 5.21
CHEMBL3416528 IC50 = 8600.0 nM 5.07
CHEMBL3416307 IC50 = 15000.0 nM 4.82
CHEMBL106 FLUCONAZOLE IC50 - -