Assay Detail
Binding
CHEMBL3420247
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HO1 in Sprague-Dawley rat spleen microsomal fraction assessed as reduction in bilirubin formation incubated for 60 mins by double beam spectrophotometry
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Tissue | Spleen |
| Subcellular | Microsomal |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Novel imidazole derivatives as heme oxygenase-1 (HO-1) and heme oxygenase-2 (HO-2) inhibitors and their cytotoxic activity in human-derived cancer cell lines.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 4.77 | 6.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3415248 | — | — | 1 | 6.05 |
| CHEMBL3415249 | — | — | 1 | 6.00 |
| CHEMBL3415255 | — | — | 1 | 4.78 |
| CHEMBL3415246 | — | — | 1 | 4.77 |
| CHEMBL3415254 | — | — | 1 | 4.75 |
| CHEMBL3415251 | — | — | 1 | 4.74 |
| CHEMBL3415256 | — | — | 1 | 4.70 |
| CHEMBL3415247 | — | — | 1 | 4.51 |
| CHEMBL3415243 | — | — | 1 | 4.47 |
| CHEMBL3415253 | — | — | 1 | 4.44 |
| CHEMBL3415250 | — | — | 1 | 4.40 |
| CHEMBL3415245 | — | — | 1 | 4.38 |
| CHEMBL3415242 | — | — | 1 | 4.37 |
| CHEMBL3415244 | — | — | 1 | 4.36 |
| CHEMBL3415252 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3415248 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL3415249 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL3415255 | — | IC50 | = | 16600.0 | nM | 4.78 |
| CHEMBL3415246 | — | IC50 | = | 16900.0 | nM | 4.77 |
| CHEMBL3415254 | — | IC50 | = | 17700.0 | nM | 4.75 |
| CHEMBL3415251 | — | IC50 | = | 18400.0 | nM | 4.74 |
| CHEMBL3415256 | — | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL3415247 | — | IC50 | = | 30900.0 | nM | 4.51 |
| CHEMBL3415243 | — | IC50 | = | 34000.0 | nM | 4.47 |
| CHEMBL3415253 | — | IC50 | = | 36300.0 | nM | 4.44 |
| CHEMBL3415250 | — | IC50 | = | 39800.0 | nM | 4.40 |
| CHEMBL3415245 | — | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL3415242 | — | IC50 | = | 42600.0 | nM | 4.37 |
| CHEMBL3415244 | — | IC50 | = | 44000.0 | nM | 4.36 |
| CHEMBL3415252 | — | IC50 | > | 100000.0 | nM | - |