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Assay Detail

CHEMBL3421140

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Binding
Inhibition of recombinant His6-tagged Pseudomonas aeruginosa PqsD expressed in Escherichia coli BL21 (deltaDE3) using anthraniloyl-CoA/beta-ketodecanoic acid as substrate assessed as HHQ formation preincubated for 10 mins followed by substrate addition by HPLC/MS-MS analysis
26
Total Activities
26
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

2-heptyl-4(1H)-quinolone synthase PqsD (CHEMBL3559673)
Type SINGLE PROTEIN
Organism Pseudomonas aeruginosa

Publication

Exploring the chemical space of ureidothiophene-2-carboxylic acids as inhibitors of the quorum sensing enzyme PqsD from Pseudomonas aeruginosa.
Sahner JH, Empting M, Kamal A, Weidel E, Groh M, Börger C, Hartmann RW.
Eur J Med Chem (2015) 96 : 14 -21
PubMed 25874327 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 5.15 6.85
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3414805 1 6.85
CHEMBL3414806 1 6.44
CHEMBL3086445 1 6.30
CHEMBL2413670 1 6.00
CHEMBL369485 1 5.70
CHEMBL3414804 1 5.57
CHEMBL3414797 1 5.42
CHEMBL3414794 1 5.30
CHEMBL3414802 1 5.21
CHEMBL3414785 1 5.16
CHEMBL3086442 1 5.16
CHEMBL3414799 1 5.12
CHEMBL3414801 1 4.94
CHEMBL3414793 1 4.88
CHEMBL3414788 1 4.86
CHEMBL3414792 1 4.72
CHEMBL3414803 1 4.71
CHEMBL3414796 1 4.67
CHEMBL3414800 1 4.62
CHEMBL3414786 1 4.54
CHEMBL3414795 1 4.52
CHEMBL3414808 1 4.50
CHEMBL3414791 1 4.50
CHEMBL3414787 1 4.50
CHEMBL3414790 1 4.47
CHEMBL3414809 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3414805 IC50 = 140.0 nM 6.85
CHEMBL3414806 IC50 = 360.0 nM 6.44
CHEMBL3086445 IC50 = 500.0 nM 6.30
CHEMBL2413670 IC50 = 1000.0 nM 6.00
CHEMBL369485 IC50 = 2000.0 nM 5.70
CHEMBL3414804 IC50 = 2700.0 nM 5.57
CHEMBL3414797 IC50 = 3800.0 nM 5.42
CHEMBL3414794 IC50 = 5000.0 nM 5.30
CHEMBL3414802 IC50 = 6200.0 nM 5.21
CHEMBL3414785 IC50 = 7000.0 nM 5.16
CHEMBL3086442 IC50 = 7000.0 nM 5.16
CHEMBL3414799 IC50 = 7500.0 nM 5.12
CHEMBL3414801 IC50 = 11500.0 nM 4.94
CHEMBL3414793 IC50 = 13200.0 nM 4.88
CHEMBL3414788 IC50 = 13800.0 nM 4.86
CHEMBL3414792 IC50 = 19000.0 nM 4.72
CHEMBL3414803 IC50 = 19400.0 nM 4.71
CHEMBL3414796 IC50 = 21600.0 nM 4.67
CHEMBL3414800 IC50 = 23700.0 nM 4.62
CHEMBL3414786 IC50 = 29000.0 nM 4.54
CHEMBL3414795 IC50 = 30300.0 nM 4.52
CHEMBL3414808 IC50 = 32000.0 nM 4.50
CHEMBL3414791 IC50 = 31900.0 nM 4.50
CHEMBL3414787 IC50 = 31300.0 nM 4.50
CHEMBL3414790 IC50 = 33700.0 nM 4.47
CHEMBL3414809 Inhibition - % -