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Assay Detail

CHEMBL3421344

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]apelin-13[Glp65,Nle75,Tyr77] from YFP epitope-tagged human APJ receptor expressed in HEK293 cell membranes incubated for 1 hr by gamma counting method
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL1628481)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

C-Terminal modifications of apelin-13 significantly change ligand binding, receptor signaling, and hypotensive action.
Murza A, Besserer-Offroy É, Côté J, Bérubé P, Longpré JM, Dumaine R, Lesur O, Auger-Messier M, Leduc R, Sarret P, Marsault É.
J Med Chem (2015) 58 : 2431 -2440
PubMed 25668242 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 8.65 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3417384 1 10.70
CHEMBL3417378 1 9.60
CHEMBL3417389 1 9.37
CHEMBL3417385 1 9.32
CHEMBL3417377 1 9.22
CHEMBL3417390 1 9.07
CHEMBL3184840 1 8.92
CHEMBL3417379 1 8.89
CHEMBL3417386 1 8.82
CHEMBL3417380 1 8.80
CHEMBL3414601 1 8.77
CHEMBL3417291 1 8.59
CHEMBL3417388 1 8.55
CHEMBL3417381 1 8.21
CHEMBL3417383 1 8.17
CHEMBL3417375 1 8.10
CHEMBL3414600 1 7.94
CHEMBL3417387 1 7.86
CHEMBL3417382 1 7.84
CHEMBL3417376 1 7.74
CHEMBL3417391 1 7.24
CHEMBL3417394 1 -
CHEMBL3417392 1 -
CHEMBL3417393 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3417384 IC50 = 0.02 nM 10.70
CHEMBL3417378 IC50 = 0.25 nM 9.60
CHEMBL3417389 IC50 = 0.43 nM 9.37
CHEMBL3417385 IC50 = 0.48 nM 9.32
CHEMBL3417377 IC50 = 0.6 nM 9.22
CHEMBL3417390 IC50 = 0.86 nM 9.07
CHEMBL3184840 IC50 = 1.2 nM 8.92
CHEMBL3417379 IC50 = 1.3 nM 8.89
CHEMBL3417386 IC50 = 1.5 nM 8.82
CHEMBL3417380 IC50 = 1.6 nM 8.80
CHEMBL3414601 IC50 = 1.7 nM 8.77
CHEMBL3417291 IC50 = 2.6 nM 8.59
CHEMBL3417388 IC50 = 2.8 nM 8.55
CHEMBL3417381 IC50 = 6.1 nM 8.21
CHEMBL3417383 IC50 = 6.7 nM 8.17
CHEMBL3417375 IC50 = 7.9 nM 8.10
CHEMBL3414600 IC50 = 11.4 nM 7.94
CHEMBL3417387 IC50 = 13.7 nM 7.86
CHEMBL3417382 IC50 = 14.3 nM 7.84
CHEMBL3417376 IC50 = 18.2 nM 7.74
CHEMBL3417391 IC50 = 57.0 nM 7.24
CHEMBL3417394 IC50 > 10000.0 nM -
CHEMBL3417392 IC50 > 10000.0 nM -
CHEMBL3417393 IC50 > 10000.0 nM -