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Assay Detail

CHEMBL3424482

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat CYP11B1 expressed in hamster fibroblast using 500 nM [3H]-11-deoxycorticosterone as substrate after 7 hrs by HPLC analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 11B1, mitochondrial (CHEMBL4970)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Identification of 4-(4-nitro-2-phenethoxyphenyl)pyridine as a promising new lead for discovering inhibitors of both human and rat 11β-Hydroxylase.
Hu Q, Kunde J, Hanke N, Hartmann RW.
Eur J Med Chem (2015) 96 : 139 -150
PubMed 25874338 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.99 6.79

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3421769 1 6.79
CHEMBL3421770 1 6.43
CHEMBL3421780 1 6.36
CHEMBL3421779 1 6.26
CHEMBL3421768 1 6.19
CHEMBL3421785 1 6.15
CHEMBL3421766 1 5.88
CHEMBL3421765 1 5.83
CHEMBL2165320 1 5.82
CHEMBL3421767 1 5.68
CHEMBL2417553 1 5.61
CHEMBL3421786 1 5.54
CHEMBL934 METYRAPONE 4.0 1 5.34
CHEMBL428780 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3421769 IC50 = 163.0 nM 6.79
CHEMBL3421770 IC50 = 370.0 nM 6.43
CHEMBL3421780 IC50 = 441.0 nM 6.36
CHEMBL3421779 IC50 = 543.0 nM 6.26
CHEMBL3421768 IC50 = 648.0 nM 6.19
CHEMBL3421785 IC50 = 706.0 nM 6.15
CHEMBL3421766 IC50 = 1307.0 nM 5.88
CHEMBL3421765 IC50 = 1493.0 nM 5.83
CHEMBL2165320 IC50 = 1500.0 nM 5.82
CHEMBL3421767 IC50 = 2083.0 nM 5.68
CHEMBL2417553 IC50 = 2440.0 nM 5.61
CHEMBL3421786 IC50 = 2849.0 nM 5.54
CHEMBL934 METYRAPONE IC50 = 4607.0 nM 5.34
CHEMBL428780 IC50 > 10000.0 nM -