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Assay Detail

CHEMBL3428554

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His6-tagged UBLCP1 (unknown origin) expressed in Escherichia coli BL21 cells using pNPP as substrate at pH 6 at 25 degC by spectrophotometric analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BL21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin-like domain-containing CTD phosphatase 1 (CHEMBL3317333)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A potent and selective inhibitor for the UBLCP1 proteasome phosphatase.
He Y, Guo X, Yu ZH, Wu L, Gunawan AM, Zhang Y, Dixon JE, Zhang ZY.
Bioorg Med Chem (2015) 23 : 2798 -2809
PubMed 25907364 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.40 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3426913 1 6.30
CHEMBL3425525 1 5.52
CHEMBL3426911 1 5.38
CHEMBL3426909 1 5.38
CHEMBL3426907 1 5.36
CHEMBL3426912 1 5.27
CHEMBL3426910 1 5.08
CHEMBL3426908 1 4.87
CHEMBL424 SALICYLIC ACID 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3426913 IC50 = 500.0 nM 6.30
CHEMBL3425525 IC50 = 3000.0 nM 5.52
CHEMBL3426911 IC50 = 4200.0 nM 5.38
CHEMBL3426909 IC50 = 4200.0 nM 5.38
CHEMBL3426907 IC50 = 4400.0 nM 5.36
CHEMBL3426912 IC50 = 5400.0 nM 5.27
CHEMBL3426910 IC50 = 8300.0 nM 5.08
CHEMBL3426908 IC50 = 13500.0 nM 4.87
CHEMBL424 SALICYLIC ACID IC50 = 66000000.0 nM -