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Assay Detail

CHEMBL3430265

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GFP-tagged human BCRP expressed in MDCK2 cells pre-incubated for 30 mins followed by Hoechst 33342 addition and further incubated for 120 mins by Hoechst 33342 accumulation assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK-II
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

HM30181 Derivatives as Novel Potent and Selective Inhibitors of the Breast Cancer Resistance Protein (BCRP/ABCG2).
Köhler SC, Wiese M.
J Med Chem (2015) 58 : 3910 -3921
PubMed 25855895 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.72 7.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3426401 1 7.19
CHEMBL3426399 1 7.14
CHEMBL3426402 1 7.10
CHEMBL3426387 1 7.10
CHEMBL3426384 1 7.00
CHEMBL3426395 1 6.97
CHEMBL3426388 1 6.93
CHEMBL3426398 1 6.93
CHEMBL488910 1 6.89
CHEMBL3426391 1 6.86
CHEMBL3426400 1 6.75
CHEMBL3426382 1 6.74
CHEMBL3426397 1 6.70
CHEMBL3426386 1 6.58
CHEMBL3426396 1 6.57
CHEMBL3426393 1 6.35
CHEMBL428402 1 6.14
CHEMBL3426394 1 4.95

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3426401 IC50 = 64.2 nM 7.19
CHEMBL3426399 IC50 = 73.0 nM 7.14
CHEMBL3426402 IC50 = 79.4 nM 7.10
CHEMBL3426387 IC50 = 78.9 nM 7.10
CHEMBL3426384 IC50 = 100.0 nM 7.00
CHEMBL3426395 IC50 = 106.0 nM 6.97
CHEMBL3426388 IC50 = 118.0 nM 6.93
CHEMBL3426398 IC50 = 117.0 nM 6.93
CHEMBL488910 IC50 = 128.0 nM 6.89
CHEMBL3426391 IC50 = 137.0 nM 6.86
CHEMBL3426400 IC50 = 180.0 nM 6.75
CHEMBL3426382 IC50 = 184.0 nM 6.74
CHEMBL3426397 IC50 = 201.0 nM 6.70
CHEMBL3426386 IC50 = 261.0 nM 6.58
CHEMBL3426396 IC50 = 269.0 nM 6.57
CHEMBL3426393 IC50 = 451.0 nM 6.35
CHEMBL428402 IC50 = 721.0 nM 6.14
CHEMBL3426394 IC50 = 11200.0 nM 4.95