Assay Detail
Functional
CHEMBL3436099
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit
1
Total Activities
1
Compounds Tested
1
Activity Types
6
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Voltage-dependent L-type calcium channel subunit alpha-1C (CHEMBL1940) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Predictive model for L-type channel inhibition: multichannel block in QT prolongation risk assessment.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 6.60 | 6.60 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| CA_SOL_CONC | CA_SOL_CONC | 10.0 | mM | — |
| DETECTION_METHOD | DETECTION_METHOD | - | — | Whole cell PC |
| HOLDING_POTENTIAL | HOLDING_POTENTIAL | - | — | -80 |
| MEAS_VOLT | MEAS_VOLT | 10.0 | mV | — |
| T1_PULSE | T1_PULSE | 0.5 | s | — |
| TEMP | TEMP | - | — | room |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1484 | NICARDIPINE | 4.0 | 1 | 6.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1484 | NICARDIPINE | IC50 | = | 250.0 | nM | 6.60 |