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Assay Detail

CHEMBL3531728

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human CYP2E1 expressed in Escherichia coli MV1304 assessed as reduction in 7-EFC O-de-ethylation activity by spectrofluorometry based double reciprocal plot
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 2E1 (CHEMBL5281)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The inactivation of human CYP2E1 by phenethyl isothiocyanate, a naturally occurring chemopreventive agent, and its oxidative bioactivation.
Yoshigae Y, Sridar C, Kent UM, Hollenberg PF.
Drug Metab Dispos (2013) 41 : 858 -869
PubMed 23371965 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kinact 1 - -
Ki 1 4.96 4.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL151649 PHENETHYLISOTHIOCYANATE 2 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL151649 PHENETHYLISOTHIOCYANATE Ki = 11000.0 nM 4.96
CHEMBL151649 PHENETHYLISOTHIOCYANATE Kinact = 0.23 /min -